FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1984896077> ?p ?o ?g. }

• W1984896077 endingPage "363" @default.
• W1984896077 startingPage "355" @default.
• W1984896077 abstract "The use of reference genes as internal controls is commonly accepted as the most appropriate normalization strategy in quantitative real-time PCR (qPCR) assays. However, there is increasing evidence that expression of many putative reference genes may be regulated by the experimental conditions, and thereby may result in an inaccurate or incorrect quantification of target gene mRNA expression. The aim of this study was to quantitatively evaluate commonly used reference genes for their suitability as a normalization factor for gene expression analyses in the intestine during development of a soybean meal (SBM)-induced intestinal inflammation (enteropathy) in Atlantic salmon. The software applications geNorm, NormFinder and BestKeeper were used to rank eight reference genes according to their stability across 80 samples from a feeding trial with sequential sampling at 10 time points following initiation of SBM exposure. Additionally, we propose a novel statistical model for estimation and ranking of reference gene stability, based on the coefficient of variation (CV) and the Fisher test. ACTB, EF1A, G6PDH and RPS20 mRNA levels displayed a time-dependent induction during development of the enteropathy. In contrast, 18S, GAPDH, RNAPOLII and HPRTI were more stably expressed during the experiment. Overall, a combination of GAPDH, RNAPOLII and HPRTI is recommended as an internal normalization factor in qPCR assays of the distal intestine of Atlantic salmon with SBM-induced enteropathy. Furthermore, we demonstrate that ignoring underlying assumptions made by normalization software may result in an inaccurate or even completely incorrect conclusion on the selection of the best reference gene(s)." @default.
• W1984896077 created "2016-06-24" @default.
• W1984896077 creator A5015111177 @default.
• W1984896077 creator A5017331327 @default.
• W1984896077 creator A5036243903 @default.
• W1984896077 creator A5047259413 @default.
• W1984896077 creator A5067928736 @default.
• W1984896077 creator A5073530658 @default.
• W1984896077 date "2011-08-01" @default.
• W1984896077 modified "2023-10-16" @default.
• W1984896077 title "Candidate reference genes for quantitative real-time PCR (qPCR) assays during development of a diet-related enteropathy in Atlantic salmon (Salmo salar L.) and the potential pitfalls of uncritical use of normalization software tools" @default.
• W1984896077 cites W1534062799 @default.
• W1984896077 cites W1577577364 @default.
• W1984896077 cites W163511390 @default.
• W1984896077 cites W1985569492 @default.
• W1984896077 cites W1987228708 @default.
• W1984896077 cites W2005174657 @default.
• W1984896077 cites W2014498146 @default.
• W1984896077 cites W2015083532 @default.
• W1984896077 cites W2016067593 @default.
• W1984896077 cites W2025492442 @default.
• W1984896077 cites W2040543471 @default.
• W1984896077 cites W2044518973 @default.
• W1984896077 cites W2045241723 @default.
• W1984896077 cites W2054458730 @default.
• W1984896077 cites W2057464020 @default.
• W1984896077 cites W2061133013 @default.
• W1984896077 cites W2077456030 @default.
• W1984896077 cites W2084711832 @default.
• W1984896077 cites W2088494800 @default.
• W1984896077 cites W2099824652 @default.
• W1984896077 cites W2100178859 @default.
• W1984896077 cites W2105135948 @default.
• W1984896077 cites W2105423509 @default.
• W1984896077 cites W2105816476 @default.
• W1984896077 cites W2110625071 @default.
• W1984896077 cites W2126835198 @default.
• W1984896077 cites W2127535576 @default.
• W1984896077 cites W2133948559 @default.
• W1984896077 cites W2136884513 @default.
• W1984896077 cites W2137581199 @default.
• W1984896077 cites W2140451732 @default.
• W1984896077 cites W2143146123 @default.
• W1984896077 cites W2145879504 @default.
• W1984896077 cites W2148927308 @default.
• W1984896077 cites W2150574926 @default.
• W1984896077 cites W2157234679 @default.
• W1984896077 cites W2163074585 @default.
• W1984896077 cites W2164095252 @default.
• W1984896077 cites W2166642834 @default.
• W1984896077 cites W2168420558 @default.
• W1984896077 cites W4241854952 @default.
• W1984896077 doi "https://doi.org/10.1016/j.aquaculture.2011.05.038" @default.
• W1984896077 hasPublicationYear "2011" @default.
• W1984896077 type Work @default.
• W1984896077 sameAs 1984896077 @default.
• W1984896077 citedByCount "62" @default.
• W1984896077 countsByYear W19848960772012 @default.
• W1984896077 countsByYear W19848960772013 @default.
• W1984896077 countsByYear W19848960772014 @default.
• W1984896077 countsByYear W19848960772015 @default.
• W1984896077 countsByYear W19848960772016 @default.
• W1984896077 countsByYear W19848960772017 @default.
• W1984896077 countsByYear W19848960772018 @default.
• W1984896077 countsByYear W19848960772019 @default.
• W1984896077 countsByYear W19848960772020 @default.
• W1984896077 countsByYear W19848960772021 @default.
• W1984896077 countsByYear W19848960772022 @default.
• W1984896077 countsByYear W19848960772023 @default.
• W1984896077 crossrefType "journal-article" @default.
• W1984896077 hasAuthorship W1984896077A5015111177 @default.
• W1984896077 hasAuthorship W1984896077A5017331327 @default.
• W1984896077 hasAuthorship W1984896077A5036243903 @default.
• W1984896077 hasAuthorship W1984896077A5047259413 @default.
• W1984896077 hasAuthorship W1984896077A5067928736 @default.
• W1984896077 hasAuthorship W1984896077A5073530658 @default.
• W1984896077 hasConcept C104317684 @default.
• W1984896077 hasConcept C126322002 @default.
• W1984896077 hasConcept C136886441 @default.
• W1984896077 hasConcept C144024400 @default.
• W1984896077 hasConcept C150194340 @default.
• W1984896077 hasConcept C19165224 @default.
• W1984896077 hasConcept C2777940460 @default.
• W1984896077 hasConcept C2778426112 @default.
• W1984896077 hasConcept C2779134260 @default.
• W1984896077 hasConcept C2779148672 @default.
• W1984896077 hasConcept C2909208804 @default.
• W1984896077 hasConcept C48023723 @default.
• W1984896077 hasConcept C505870484 @default.
• W1984896077 hasConcept C54355233 @default.
• W1984896077 hasConcept C69991583 @default.
• W1984896077 hasConcept C70721500 @default.
• W1984896077 hasConcept C71924100 @default.
• W1984896077 hasConcept C86803240 @default.
• W1984896077 hasConceptScore W1984896077C104317684 @default.
• W1984896077 hasConceptScore W1984896077C126322002 @default.
• W1984896077 hasConceptScore W1984896077C136886441 @default.
• W1984896077 hasConceptScore W1984896077C144024400 @default.

• next