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- W1984910326 abstract "Abstract In order to study microbial peptidase activities under cheese conditions, two different substrate models were studied: renneted skim milk and Grana cheese paste. The substrates were mixed with crude cell extracts each of 54 Lb. casei strains, isolated from Grana cheese, at various stages of ripening, from four different factories and incubated for 10 days. Electrophoretic analysis of undigested proteins, the high correlation coefficient between the soluble nitrogen fraction (OPA assay) and the total amino acids released in the models demonstrated a wide distribution of intense peptidolytic activity among the strains; no strain showed detectable proteolytic activity. Statistical analysis of the normalized concentrations of the acids amino released by each crude extract permitted the selection of the Lb. casei strains studied according to their potential ability to produce the proper amino acid pattern for (Grana) cheese ripening. Moreover, analysis of the whole data indicated that Grana cheese paste was the most suitable for studies on the proteolytic effect of lactic acid bacteria in Grana cheese." @default.
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- W1984910326 date "1996-10-01" @default.
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- W1984910326 title "Substrate models for studies on peptidolytic activity of Lactobacillus casei" @default.
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- W1984910326 doi "https://doi.org/10.1016/s0958-6946(96)00026-x" @default.
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