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• W1984927746 abstract "In vitro methods to evaluate antibacterial activity were used with contact lenses bearing levofloxacin-loaded liposomes developed for the prevention and treatment of bacterial ocular infections such as keratitis. Levofloxacin was incorporated into liposomes before these intact liposomes were immobilized onto the surfaces of soft contact lenses using a multilayer immobilization strategy. The release of levofloxacin from contact lenses bearing 2, 5, and 10 layers of liposomes into a saline buffer at 37 degrees C was monitored by fluorescence. The levofloxacin release, as a function of time, was described by a mechanism taking into account two independent first-order kinetic models. The total release of levofloxacin from the contact lenses was completed within 6 days. The release of levofloxacin from contact lenses bearing 10 layers of liposomes and subsequently soaked overnight in a levofloxacin solution was also studied and compare to that of dried contact lenses without any chemical modification rehydrated in a levofloxacin solution. The antibacterial activity of the liposome-coated contact lenses against Staphylococcus aureus was evaluated by measuring (i) the diameters of the inhibition zone on an agar plate and (ii) the optical density using a broth assay. The liposome-coated lenses showed an antibacterial activity both on agar and in broth following 24 h. When initial bacteria inocula were equal or below 10(6) CFU/mL, all the bacteria were inhibited within 2 h. When using initial bacteria inocula of 10(8) CFU/mL, an initial burst release provided by soaking the liposomal lenses was required for the first hours to inhibit bacteria growth." @default.
• W1984927746 created "2016-06-24" @default.
• W1984927746 creator A5046513064 @default.
• W1984927746 creator A5073272142 @default.
• W1984927746 creator A5089263361 @default.
• W1984927746 date "2007-09-01" @default.
• W1984927746 modified "2023-10-04" @default.
• W1984927746 title "Antibacterial Activity of Contact Lenses Bearing Surface-Immobilized Layers of Intact Liposomes Loaded With Levofloxacin" @default.
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• W1984927746 doi "https://doi.org/10.1002/jps.20871" @default.
• W1984927746 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17541976" @default.
• W1984927746 hasPublicationYear "2007" @default.
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