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- W1984964562 abstract "Abstract: We have applied the 19F-nuclear magnetic resonance (NMR) calcium indicator 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N′,N′-tetraacetic acid (5FBAPTA) to the measurement of the free intracellular calcium concentration ([Ca2+] i) in superfused brain slices. A mean ± SD control value of 380 ± 71 nM(n = 18) was obtained at 37°C using 2.4 mM extracellular Ca2+. Subcellular fractionation studies using [3 H] 5FBAPTA showed that after loading of its tetraac-etoxymethyl ester, ∼55% was de-esterified, with the other 45% remaining as the tetraester bound to membranes. Of the de-esterified 5FBAPTA, >90% was in the cytosolic fractions, with >1% in the mitochondria or microsomes. The NMR-visible de-esterified 5FBAPTA slowly disappeared from the tissue with a t1/2 of 4 h. A time course after loading confirmed that the calculated [Ca2+], was constant over a 5-h period, although the scatter of individual results was ±20%. The [Ca2+]; was increased by a high extracellular K+ concentration ([K+]e), by a low extracellular concentration of Na+, and by the calcium ionophore A23187. On recovery from high [K+]e. the [Ca2+]i“overshot” to values lower than the original control value. The [Ca2+]i was surprisingly resistant to changes in extracellular Ca2+ concentration." @default.
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- W1984964562 date "1990-09-01" @default.
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- W1984964562 title "Use of 1,2-Bis(2-Amino-5-Fluorophenoxy)ethane-N,N,N',N'-Tetraacetic Acid (5FBAPTA) in the Measurement of Free Intracellular Calcium in the Brain by<sup>19</sup>F-Nuclear Magnetic Resonance Spectroscopy" @default.
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- W1984964562 doi "https://doi.org/10.1111/j.1471-4159.1990.tb04573.x" @default.
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