FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985017503> ?p ?o ?g. }

• W1985017503 endingPage "4640" @default.
• W1985017503 startingPage "4633" @default.
• W1985017503 abstract "In this work, we evaluate the incorporation of an ultralow flow interface for coupling capillary electrophoresis (CE) and mass spectrometry (MS), in combination with reversed-phase high-pressure liquid chromatography (HPLC) fractionation as an alternate workflow for quantitative proteomics. Proteins, extracted from a SILAC (stable isotope labeling by amino acids in cell culture) labeled and an unlabeled yeast strain were mixed and digested enzymatically in solution. The resulting peptides were fractionated using RP-HPLC and analyzed by CE–MS yielding a total of 28 538 quantified peptides that correspond to 3 272 quantified proteins. CE–MS analysis was performed using a neutral capillary coating, providing the highest separation efficiency at ultralow flow conditions (<10 nL/min). Moreover, we were able to demonstrate that CE–MS is a powerful method for the identification of low-abundance modified peptides within the same sample. Without any further enrichment strategies, we succeeded in quantifying 1 371 phosphopeptides present in the CE–MS data set and found 49 phosphopeptides to be differentially regulated in the two yeast strains. Including acetylation, phosphorylation, deamidation, and oxidized forms, a total of 8 106 modified peptides could be identified in addition to 33 854 unique peptide sequences found. The work presented here shows the first quantitative proteomics approach that combines SILAC labeling with CE–MS analysis." @default.
• W1985017503 created "2016-06-24" @default.
• W1985017503 creator A5002815807 @default.
• W1985017503 creator A5046511169 @default.
• W1985017503 creator A5064428088 @default.
• W1985017503 creator A5069412131 @default.
• W1985017503 creator A5090585459 @default.
• W1985017503 date "2015-04-16" @default.
• W1985017503 modified "2023-09-27" @default.
• W1985017503 title "Quantitative Proteomics Using Ultralow Flow Capillary Electrophoresis–Mass Spectrometry" @default.
• W1985017503 cites W1897299284 @default.
• W1985017503 cites W1968403457 @default.
• W1985017503 cites W1970696723 @default.
• W1985017503 cites W1975086142 @default.
• W1985017503 cites W1975186375 @default.
• W1985017503 cites W1981515958 @default.
• W1985017503 cites W1981561723 @default.
• W1985017503 cites W1982607050 @default.
• W1985017503 cites W1985476025 @default.
• W1985017503 cites W1988005933 @default.
• W1985017503 cites W1998533111 @default.
• W1985017503 cites W2007600529 @default.
• W1985017503 cites W2012157561 @default.
• W1985017503 cites W2012842649 @default.
• W1985017503 cites W2013947447 @default.
• W1985017503 cites W2014824541 @default.
• W1985017503 cites W2016783238 @default.
• W1985017503 cites W2018236745 @default.
• W1985017503 cites W2019297097 @default.
• W1985017503 cites W2041214786 @default.
• W1985017503 cites W2046001002 @default.
• W1985017503 cites W2048207474 @default.
• W1985017503 cites W2050633231 @default.
• W1985017503 cites W2055979718 @default.
• W1985017503 cites W2063859419 @default.
• W1985017503 cites W2063912727 @default.
• W1985017503 cites W2067519806 @default.
• W1985017503 cites W2067978623 @default.
• W1985017503 cites W2068398015 @default.
• W1985017503 cites W2084058384 @default.
• W1985017503 cites W2084287944 @default.
• W1985017503 cites W2087442402 @default.
• W1985017503 cites W2089332469 @default.
• W1985017503 cites W2093507952 @default.
• W1985017503 cites W2099255819 @default.
• W1985017503 cites W2099839305 @default.
• W1985017503 cites W2103023204 @default.
• W1985017503 cites W2163310011 @default.
• W1985017503 cites W2318027074 @default.
• W1985017503 cites W2318267434 @default.
• W1985017503 cites W2329699243 @default.
• W1985017503 cites W2331314753 @default.
• W1985017503 cites W2412786668 @default.
• W1985017503 cites W4243804942 @default.
• W1985017503 doi "https://doi.org/10.1021/acs.analchem.5b00312" @default.
• W1985017503 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4423236" @default.
• W1985017503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25839223" @default.
• W1985017503 hasPublicationYear "2015" @default.
• W1985017503 type Work @default.
• W1985017503 sameAs 1985017503 @default.
• W1985017503 citedByCount "48" @default.
• W1985017503 countsByYear W19850175032015 @default.
• W1985017503 countsByYear W19850175032016 @default.
• W1985017503 countsByYear W19850175032017 @default.
• W1985017503 countsByYear W19850175032018 @default.
• W1985017503 countsByYear W19850175032019 @default.
• W1985017503 countsByYear W19850175032020 @default.
• W1985017503 countsByYear W19850175032021 @default.
• W1985017503 countsByYear W19850175032022 @default.
• W1985017503 countsByYear W19850175032023 @default.
• W1985017503 crossrefType "journal-article" @default.
• W1985017503 hasAuthorship W1985017503A5002815807 @default.
• W1985017503 hasAuthorship W1985017503A5046511169 @default.
• W1985017503 hasAuthorship W1985017503A5064428088 @default.
• W1985017503 hasAuthorship W1985017503A5069412131 @default.
• W1985017503 hasAuthorship W1985017503A5090585459 @default.
• W1985017503 hasBestOaLocation W19850175031 @default.
• W1985017503 hasConcept C104317684 @default.
• W1985017503 hasConcept C104397665 @default.
• W1985017503 hasConcept C112979427 @default.
• W1985017503 hasConcept C162356407 @default.
• W1985017503 hasConcept C181199279 @default.
• W1985017503 hasConcept C184185385 @default.
• W1985017503 hasConcept C185592680 @default.
• W1985017503 hasConcept C202157981 @default.
• W1985017503 hasConcept C2779281246 @default.
• W1985017503 hasConcept C2780784506 @default.
• W1985017503 hasConcept C2900643 @default.
• W1985017503 hasConcept C31827203 @default.
• W1985017503 hasConcept C34905852 @default.
• W1985017503 hasConcept C43617362 @default.
• W1985017503 hasConcept C46111723 @default.
• W1985017503 hasConcept C55493867 @default.
• W1985017503 hasConcept C73090800 @default.
• W1985017503 hasConcept C80311884 @default.
• W1985017503 hasConcept C95986675 @default.
• W1985017503 hasConceptScore W1985017503C104317684 @default.
• W1985017503 hasConceptScore W1985017503C104397665 @default.

• next