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- W1985049569 abstract "Immunogold staining techniques were applied to study the adsorption of fibrinogen and albumin on polymer surfaces. Colloidal gold particles were coated with antibodies (immunogold) and used to stain protein molecules adsorbed on surfaces. The two-dimensional distribution of immunogold particles was visualized by scanning electron microscopy. Since immunogold staining relies on antigen-antibody reactions, the distribution of fibrinogen and albumin on polymer surfaces could be observed even when both proteins were adsorbed simultaneously. The specific reaction also allows investigation of the change in orientation or conformation of one protein in the presence of the others. The surface distribution of fibrinogen and albumin was also examined on polymer surfaces which were exposed to blood in a canine ex vivo experiment. It was found that the sites where thrombi had embolized contained much less fibrinogen and albumin on them compared to portions of the surface where thrombi had not yet formed." @default.
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- W1985049569 date "1986-05-01" @default.
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- W1985049569 title "A new approach to study adsorbed proteins on biomaterials: Immunogold staining" @default.
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- W1985049569 doi "https://doi.org/10.1016/0021-9797(86)90019-6" @default.
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