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- W1985090529 abstract "Since the traditional approach to optimization of biological systems based on the single variable search technique is incapable of detecting the true optimum when a number of chemicals are used together as inducers, the Evolutionary Operation (EVOP)-factorial design technique was applied successfully to optimize the concentrations of vitamin (biotin), metal ion (CaCl2) and plant hormone (NAA) for maximizing enzyme (protease) production by Rhizopus oryzae (RO IIT RB-13, NRRL-21498) in solid state fermentation. Addition of inducers to an optimal level (biotin-2.5 ppm, CaCl2-20 ppm and NAA-12.5 ppm) resulted in a ∼ 1.9-fold increase in protease production." @default.
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- W1985090529 date "1999-01-01" @default.
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- W1985090529 title "Optimization of n variable biological experiments by evolutionary operation-factorial design technique" @default.
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- W1985090529 doi "https://doi.org/10.1016/s1389-1723(99)89017-3" @default.
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