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• W1985136427 endingPage "509" @default.
• W1985136427 startingPage "499" @default.
• W1985136427 abstract "Main inborn errors of metabolism diagnosable through uracil (Ura) analysis and the therapeutic monitoring of toxic 5-fluorouracil (5FU) in dihydro pyrimidine dehydrogenase (DPD) deficient patients require a sensitive, reproducible, selective and accurate method. In this work, an artificial receptor in the format of molecularly imprinted polymer (MIP) brush 'grafted to' the surface of sol-gel immobilized on cost-effective homemade solid-phase microextraction (SPME) fibers, individually imprinted with either of Ura and 5FU, was used in combination with a voltammetric sensor duly modified with the same MIP. This combination provided up to 10- and 8.4-fold preconcentrations of Ura and 5FU, respectively, which was more than sufficient for achieving stringent detection limits in the primitive diagnosis of uracil disorders and fluoropyrimidine toxicity in DPD-deficient patients. The proposed method permits the assessment of Ura and 5FU plasma concentrations with detection limits pf 0.0245 and 0.0484 ng mL(-1) (RSD = 1.0-2.5%, S/N = 3), respectively, without any problems of non-specific false-positives and cross-reactivities in complicated matrices of biological samples." @default.
• W1985136427 created "2016-06-24" @default.
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• W1985136427 creator A5076710514 @default.
• W1985136427 creator A5076876181 @default.
• W1985136427 date "2009-05-01" @default.
• W1985136427 modified "2023-09-23" @default.
• W1985136427 title "Ultratrace analysis of uracil and 5-fluorouracil by molecularly imprinted polymer brushes grafted to silylated solid-phase microextraction fiber in combination with complementary molecularly imprinted polymer-based sensor" @default.
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• W1985136427 doi "https://doi.org/10.1002/bmc.1145" @default.
• W1985136427 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19101922" @default.
• W1985136427 hasPublicationYear "2009" @default.
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