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- W1985180589 abstract "Inorganic pyrophosphate elaboration by articular cartilage may favor calcium pyrophosphate dihydrate crystal deposition. Frequently crystal deposits form in persons affected with metabolic diseases. The cartilage organ culture system was used to model these metabolic conditions while measuring the influence on extracellular pyrophosphate elaboration. Alterations of ambient pH, thyroid stimulating hormone levels, and parathyroid hormone levels did not change pyrophosphate accumulation in the media. However, subphysiologic ambient calcium concentrations (25, 100, 500 microM) increased pyrophosphate accumulation about chondrocytes 3- to 10-fold. Low calcium also induced release of [14C]adenine-labeled nucleotides from chondrocytes, potential substrates for generation of extracellular pyrophosphate by ectoenzymes. Exposing cartilage to 10% fetal bovine serum also enhanced by 50% the egress of inorganic pyrophosphate from the tissue." @default.
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- W1985180589 date "1989-08-01" @default.
- W1985180589 modified "2023-09-23" @default.
- W1985180589 title "Stimulation of inorganic pyrophosphate elaboration by cultured cartilage and chondrocytes" @default.
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- W1985180589 doi "https://doi.org/10.1016/0003-9861(89)90233-6" @default.
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