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• W1985180922 abstract "Structural and functional studies of membrane proteins have been severely hampered by difficulties in producing sufficient quantities of properly folded protein products. It is well established that cell-based expression of membrane proteins is generally problematic and frequently results in low yield, cell toxicity, protein aggregation and misfolding. Owing to its inherent open nature, cell-free protein expression has become a highly promising tool for the fast and efficient production of these difficult-to-express proteins. Here we review the most recent advances in this field, underscoring the potentials and weaknesses of the newly developed approaches and place specific emphasis on the use of nanolipoprotein particles (NLPs or nanodiscs). Structural and functional studies of membrane proteins have been severely hampered by difficulties in producing sufficient quantities of properly folded protein products. It is well established that cell-based expression of membrane proteins is generally problematic and frequently results in low yield, cell toxicity, protein aggregation and misfolding. Owing to its inherent open nature, cell-free protein expression has become a highly promising tool for the fast and efficient production of these difficult-to-express proteins. Here we review the most recent advances in this field, underscoring the potentials and weaknesses of the newly developed approaches and place specific emphasis on the use of nanolipoprotein particles (NLPs or nanodiscs). any of various proteins that combine with a lipid to form a lipoprotein and are a constituent of cholesterol-carrying molecules. membrane formed by joining lipid-monolayer-encased aqueous droplets that are submerged in an oil–lipid mixture. concentration of surfactants above which micelles are spontaneously formed. nuclear or cytoplasmic aggregate of stainable substances, usually proteins. a closed lipid vesicle surrounding an aqueous interior. a discoidal particle of defined and controllable size formed by a phospholipid bilayer planar segment surrounded by a protein coat (scaffold protein). the transformation of a thermodynamic system from one phase to another. At the phase-transition point, physical properties may undergo abrupt change. a liposome into which one or more proteins have been inserted, usually by artificial means. an area of biological research that combines science and engineering in order to design and build (‘synthesize’) novel biological functions and systems. protein complex associated with the translocation of nascent polypeptides across membranes." @default.
• W1985180922 created "2016-06-24" @default.
• W1985180922 creator A5002350338 @default.
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• W1985180922 date "2009-08-01" @default.
• W1985180922 modified "2023-10-09" @default.
• W1985180922 title "Membrane protein expression: no cells required" @default.
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• W1985180922 doi "https://doi.org/10.1016/j.tibtech.2009.05.005" @default.
• W1985180922 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19616329" @default.
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