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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985187896> ?p ?o ?g. }

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• W1985187896 abstract "A truncated molecule containing the N-terminal 183 amino acid residues of CD4 (sCD4-183) has been produced in Escherichia coli at high levels, using the trp promoter and an AT-rich ribosome binding site to direct expression in a pBR322-derived vector. A culture has been selected which allows large-scale fermentation and production of this material as an insoluble inclusion body protein. Procedures which solubilize, refold, and purify sCD4-183 have been developed. The purified sCD4-183 binds gp120 in solution and blocks human immunodeficiency virus (HIV) infection of human peripheral blood lymphocytes in vitro." @default.
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• W1985187896 date "1990-04-01" @default.
• W1985187896 modified "2023-10-18" @default.
• W1985187896 title "<i>Escherichia coli</i>Expression, Purification, and Biological Activity of a Truncated Soluble CD4" @default.
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• W1985187896 doi "https://doi.org/10.1089/aid.1990.6.465" @default.
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