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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985208790> ?p ?o ?g. }

• W1985208790 endingPage "2914" @default.
• W1985208790 startingPage "2909" @default.
• W1985208790 abstract "Replication of complementary-strand DNA in filamentous phages is initiated by a primer RNA that is synthesized at the minus-strand origin on the viral single-stranded DNA by Escherichia coli RNA polymerase holoenzyme containing the σ 70 subunit. We have demonstrated that the affinity of RNA polymerase in vitro to the origin is about 16-fold higher than that to the lac UV5 promoter. We have also shown that the temperature dependence of the primer RNA synthesis is much lower than that of lac UV5 transcription. The high affinity of RNA polymerase to the origin depends on the single strandedness of the “−10 region.” A nucleotide sequence of the nontemplate strand in the −10 region was found to be important for the function, but that of the template strand was not. These observations suggest that σ 70 subunit directly interacts with the single-stranded nontemplate strand containing adenine residue(s) at the −10 region of promoter." @default.
• W1985208790 created "2016-06-24" @default.
• W1985208790 creator A5054985030 @default.
• W1985208790 creator A5075608631 @default.
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• W1985208790 date "1997-04-01" @default.
• W1985208790 modified "2023-10-16" @default.
• W1985208790 title "Minus-strand origin of filamentous phage versus transcriptional promoters in recognition of RNA polymerase" @default.
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• W1985208790 doi "https://doi.org/10.1073/pnas.94.7.2909" @default.
• W1985208790 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/20296" @default.
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• W1985208790 hasPublicationYear "1997" @default.
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