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- W1985231738 abstract "A FIA method has been developed and fully characterized for the simultaneous detection of ammonia and glutamine in culture media. Ammonia detection is based upon a chemical method and does not require an electrode. This species diffuses across a hydrophobic porous membrane into an indicator solution, the absorbance of which is continuously monitored using a LED and a phototransistor. Glutamine was determined by a difference method in which ammonia was measured before and after passage through a module containing immobilized glutaminase. Very good linearity was observed in the range 0–4 mM for both species. Total analysis time was 22 min. This FIA method was used to accurately monitor ammonia and glutamine on line for over 600 h for a hybridoma culture performed in a hollow-fiber reactor. FIA measurements were in good agreement with off-line measurements. No instrument failures occurred, thanks to systematic cleaning and maintenance procedures. This FIA method is a very attractive tool for the monitoring, and possibly control, of continuous cultures over extended periods." @default.
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- W1985231738 date "1996-10-18" @default.
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- W1985231738 title "On-line simultaneous monitoring of ammonia and glutamine in a hollow-fiber reactor using flow injection analysis" @default.
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- W1985231738 doi "https://doi.org/10.1016/0168-1656(96)01558-1" @default.
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