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• W1985231967 abstract "RNA interference (RNAi) is a powerful method to inhibit gene expression in a sequence specific manner. Here, we described the development of RNAi by micro-injection of double-stranded RNA (dsRNA) in the pea aphid Acyrthosiphon pisum. Injection of dsRNA into whole aphid body induced the silencing of two marker genes with different expression patterns: the ubiquitously expressed Ap-crt genes encoding a calreticulin and the gut specific Ap-cath-L gene encoding a cathepsin-L. Time-course analysis of the silencing showed similar temporal patterns for both genes: inhibition started at 1 day after injection, reached its maximum at 5 days and stopped at 7 days. A comparable 40% decrease of gene expression was observed for Ap-crt and Ap-cath-L. The pea aphid is the first Hemipteran insect for which genome sequence will be available soon. The gene knockdown technique developed in this study will be an essential post-genomic tool for further investigations in aphidology." @default.
• W1985231967 created "2016-06-24" @default.
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• W1985231967 date "2007-01-01" @default.
• W1985231967 modified "2023-10-09" @default.
• W1985231967 title "Gene knockdown by RNAi in the pea aphid Acyrthosiphon pisum" @default.
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• W1985231967 doi "https://doi.org/10.1186/1472-6750-7-63" @default.
• W1985231967 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2075495" @default.
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