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- W1985249253 abstract "ADSL deficiency is a disorder of purine metabolism with a broad clinical spectrum. A rapid and simple HPLC-based assay to measure ADSL activity in erythrocytes was developed. The suitability of DBSs was assessed. ADSL activity was measured in erythrocyte lysates and DBS using succinyl-AMP as the substrate. Detection and quantification were performed using isocratic ion-pairing reversed-phase HPLC with UV-detection. Reference values in erythrocyte lysates were established. The intra- and interassay variations were 2% and 8%, respectively. ADSL deficiency was easily recognized. ADSL activity in DBS was highly unstable, disqualifying DBS for diagnostic procedures." @default.
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- W1985249253 date "2011-11-01" @default.
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- W1985249253 title "An HPLC-Based Assay of Adenylosuccinate Lyase in Erythrocytes" @default.
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- W1985249253 doi "https://doi.org/10.1080/15257770.2011.621008" @default.
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