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- W1985252375 abstract "A method for the identification and quantification of canonic and isoforms of phytochelatins (PCs) from Chlamydomonas reinhardtii was developed. After disulfide reduction with tris(2-carboxyethyl)phosphine (TCEP) PCs were derivatized with ferrocenecarboxylic acid (2-maleimidoyl)ethylamide (FMEA) in order to avoid oxidation of the free thiol functions during analysis. Liquid chromatography (LC) coupled to electrospray mass spectrometry (ESI-MS) and inductively coupled plasma-mass spectrometry (ICP-MS) was used for rapid and quantitative analysis of the precolumn derivatized PCs. PC2−4, CysGSH, CysPC2−4, CysPC2desGly, CysPC2Glu and CysPC2Ala were determined in the algal samples depending on the exposure of the cells to cadmium ions." @default.
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- W1985252375 date "2010-01-01" @default.
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- W1985252375 title "Quantification of Phytochelatins in Chlamydomonas reinhardtii using ferrocene-based derivatization" @default.
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- W1985252375 doi "https://doi.org/10.1039/c005014h" @default.
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