FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985252600> ?p ?o ?g. }

• W1985252600 endingPage "1746" @default.
• W1985252600 startingPage "1741" @default.
• W1985252600 abstract "Fluorescence tends to produce the lowest detection limits for most forms of capillary electrophoresis. Two issues have discouraged its use in capillary isoelectric focusing. The first issue is fluorescent labeling of proteins. Most labeling reagents react with lysine residues and convert the cationic residue to a neutral or anionic product. At best, these reagents perturb the isoelectric point of the protein. At worse, they convert each protein into hundreds of different fluorescent products that confound analysis. The second issue is the large background signal generated by impurities within commercial ampholytes. This background signal is particularly strong when excited in the blue portion of the spectrum, which is required by many common fluorescent labeling reagents. This paper addresses these issues. For labeling, we employ Chromeo P540, which is a fluorogenic reagent that converts cationic lysine residues to cationic fluorescent products. The reaction products are excited in the green, which reduces the background signal generated by impurities present within the ampholytes. To further reduce the background signal, we photobleach ampholytes with high-power photodiodes. Photobleaching reduced the noise in the ampholyte blank by an order of magnitude. Isoelectric focusing performed with photobleached pH 3−10 ampholytes produced concentration detection limits of 270 ± 25 fM and mass detection limits of 150 ± 15 zmol for Chromeo P540 labeled β-lactoglobulin. Concentration detection limits were 520 ± 40 fM and mass detection limits were 310 ± 30 zmol with pH 4−8 ampholytes. A homogenate was prepared from a Barrett’s esophagus cell line and separated by capillary isoelectric focusing, reproducibly generating dozens of peaks. The sample taken for the separation was equal to the labeled protein homogenate from three cells." @default.
• W1985252600 created "2016-06-24" @default.
• W1985252600 creator A5049743383 @default.
• W1985252600 creator A5054233368 @default.
• W1985252600 creator A5069298550 @default.
• W1985252600 creator A5086220920 @default.
• W1985252600 date "2009-02-10" @default.
• W1985252600 modified "2023-09-27" @default.
• W1985252600 title "Femtomolar Concentration Detection Limit and Zeptomole Mass Detection Limit for Protein Separation by Capillary Isoelectric Focusing and Laser-Induced Fluorescence Detection" @default.
• W1985252600 cites W1481033376 @default.
• W1985252600 cites W1560140528 @default.
• W1985252600 cites W1583023784 @default.
• W1985252600 cites W1973010976 @default.
• W1985252600 cites W1976045622 @default.
• W1985252600 cites W1977260380 @default.
• W1985252600 cites W1978277580 @default.
• W1985252600 cites W1979811473 @default.
• W1985252600 cites W1988556197 @default.
• W1985252600 cites W1989337834 @default.
• W1985252600 cites W1991961670 @default.
• W1985252600 cites W1995845413 @default.
• W1985252600 cites W1996979312 @default.
• W1985252600 cites W1996991881 @default.
• W1985252600 cites W1998324825 @default.
• W1985252600 cites W1999480433 @default.
• W1985252600 cites W2004040973 @default.
• W1985252600 cites W2007970698 @default.
• W1985252600 cites W2009369287 @default.
• W1985252600 cites W2009739420 @default.
• W1985252600 cites W2011369587 @default.
• W1985252600 cites W2011550451 @default.
• W1985252600 cites W2018511614 @default.
• W1985252600 cites W2022111622 @default.
• W1985252600 cites W2024596539 @default.
• W1985252600 cites W2025062685 @default.
• W1985252600 cites W2032870400 @default.
• W1985252600 cites W2037799109 @default.
• W1985252600 cites W2040347730 @default.
• W1985252600 cites W2040599329 @default.
• W1985252600 cites W2046557218 @default.
• W1985252600 cites W2048215036 @default.
• W1985252600 cites W2049602383 @default.
• W1985252600 cites W2056601391 @default.
• W1985252600 cites W2057905815 @default.
• W1985252600 cites W2059408526 @default.
• W1985252600 cites W2063192182 @default.
• W1985252600 cites W2065289168 @default.
• W1985252600 cites W2065860405 @default.
• W1985252600 cites W2075734514 @default.
• W1985252600 cites W2078828436 @default.
• W1985252600 cites W2080367104 @default.
• W1985252600 cites W2080399775 @default.
• W1985252600 cites W2088534740 @default.
• W1985252600 cites W2089575266 @default.
• W1985252600 cites W2089774788 @default.
• W1985252600 cites W2089954489 @default.
• W1985252600 cites W2111813941 @default.
• W1985252600 cites W2129006023 @default.
• W1985252600 cites W2133861042 @default.
• W1985252600 cites W2140613691 @default.
• W1985252600 cites W2149096965 @default.
• W1985252600 cites W2151746407 @default.
• W1985252600 cites W2161497394 @default.
• W1985252600 cites W2166998713 @default.
• W1985252600 cites W4246041572 @default.
• W1985252600 cites W4248115912 @default.
• W1985252600 cites W4250745716 @default.
• W1985252600 doi "https://doi.org/10.1021/ac8025948" @default.
• W1985252600 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2765481" @default.
• W1985252600 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19206532" @default.
• W1985252600 hasPublicationYear "2009" @default.
• W1985252600 type Work @default.
• W1985252600 sameAs 1985252600 @default.
• W1985252600 citedByCount "53" @default.
• W1985252600 countsByYear W19852526002012 @default.
• W1985252600 countsByYear W19852526002013 @default.
• W1985252600 countsByYear W19852526002014 @default.
• W1985252600 countsByYear W19852526002016 @default.
• W1985252600 countsByYear W19852526002017 @default.
• W1985252600 countsByYear W19852526002018 @default.
• W1985252600 countsByYear W19852526002020 @default.
• W1985252600 countsByYear W19852526002021 @default.
• W1985252600 crossrefType "journal-article" @default.
• W1985252600 hasAuthorship W1985252600A5049743383 @default.
• W1985252600 hasAuthorship W1985252600A5054233368 @default.
• W1985252600 hasAuthorship W1985252600A5069298550 @default.
• W1985252600 hasAuthorship W1985252600A5086220920 @default.
• W1985252600 hasBestOaLocation W19852526002 @default.
• W1985252600 hasConcept C113196181 @default.
• W1985252600 hasConcept C119128265 @default.
• W1985252600 hasConcept C121332964 @default.
• W1985252600 hasConcept C178790620 @default.
• W1985252600 hasConcept C181199279 @default.
• W1985252600 hasConcept C183882617 @default.
• W1985252600 hasConcept C185592680 @default.
• W1985252600 hasConcept C2900643 @default.
• W1985252600 hasConcept C31332276 @default.
• W1985252600 hasConcept C40875361 @default.

• next