FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985265215> ?p ?o ?g. }

• W1985265215 endingPage "13648" @default.
• W1985265215 startingPage "13639" @default.
• W1985265215 abstract "Ionized nucleobases are required for folding, conformational switching, or catalysis in a number of functional RNAs. A common strategy to study these sites employs nucleoside analogues with perturbed pKa, but the interpretation of these studies is often complicated by the chemical modification introduced, in particular modifications that add, remove, or translocate hydrogen bonding groups in addition to perturbing pKa values. In the present study we present a series of fluorine substituted adenosine analogues that produce large changes in N1 pKa values with minimal structural perturbation. These analogues include fluorine for hydrogen substitutions in the adenine ring of adenosine and 7-deaza-adenosine with resulting N1 pKa values spanning more than 4 pKa units. To demonstrate the utility of these analogues we have conducted a nucleotide analogue interference mapping (NAIM) study on a self-ligating construct of the Varkud Satellite (VS) ribozyme. We find that each of the analogues is readily incorporated by T7 RNA polymerase and produces fully active transcripts when substituted at the majority of sites. Strong interferences are observed for three sites known to be critical for VS ribozyme function, most notably A756. Substitutions at A756 lead to slight enhancements in activity for elevated pKa analogues and dramatic interferences in activity for reduced pKa analogues, supporting the proposed catalytic role for this base. The structural similarity of these analogues, combined with their even incorporation and selective interference, provides an improved method for identifying sites of adenosine protonation in a variety of systems." @default.
• W1985265215 created "2016-06-24" @default.
• W1985265215 creator A5039902482 @default.
• W1985265215 creator A5066873256 @default.
• W1985265215 date "2008-09-20" @default.
• W1985265215 modified "2023-09-27" @default.
• W1985265215 title "Fluorine Substituted Adenosines As Probes of Nucleobase Protonation in Functional RNAs" @default.
• W1985265215 cites W1487200225 @default.
• W1985265215 cites W1550887012 @default.
• W1985265215 cites W1592776763 @default.
• W1985265215 cites W164080038 @default.
• W1985265215 cites W175036826 @default.
• W1985265215 cites W1964066172 @default.
• W1985265215 cites W1968282235 @default.
• W1985265215 cites W1969669203 @default.
• W1985265215 cites W1972708527 @default.
• W1985265215 cites W1974000520 @default.
• W1985265215 cites W1974091885 @default.
• W1985265215 cites W1976027096 @default.
• W1985265215 cites W1979587445 @default.
• W1985265215 cites W1987287995 @default.
• W1985265215 cites W1987643271 @default.
• W1985265215 cites W1989579221 @default.
• W1985265215 cites W2003201962 @default.
• W1985265215 cites W2014449612 @default.
• W1985265215 cites W2014733573 @default.
• W1985265215 cites W2021333801 @default.
• W1985265215 cites W2031464203 @default.
• W1985265215 cites W2049289210 @default.
• W1985265215 cites W2050703512 @default.
• W1985265215 cites W2055049730 @default.
• W1985265215 cites W2056698714 @default.
• W1985265215 cites W2058174295 @default.
• W1985265215 cites W2058565352 @default.
• W1985265215 cites W2060192357 @default.
• W1985265215 cites W2063462908 @default.
• W1985265215 cites W2066891633 @default.
• W1985265215 cites W2068565223 @default.
• W1985265215 cites W2069503670 @default.
• W1985265215 cites W2073459948 @default.
• W1985265215 cites W2074312400 @default.
• W1985265215 cites W2077565827 @default.
• W1985265215 cites W2082751764 @default.
• W1985265215 cites W2083341124 @default.
• W1985265215 cites W2084215883 @default.
• W1985265215 cites W2085916364 @default.
• W1985265215 cites W2092221199 @default.
• W1985265215 cites W2093765227 @default.
• W1985265215 cites W2120115869 @default.
• W1985265215 cites W2121287580 @default.
• W1985265215 cites W2121670483 @default.
• W1985265215 cites W2124017549 @default.
• W1985265215 cites W2125158708 @default.
• W1985265215 cites W2131468364 @default.
• W1985265215 cites W2135058126 @default.
• W1985265215 cites W2147681005 @default.
• W1985265215 cites W2163980854 @default.
• W1985265215 cites W2165759309 @default.
• W1985265215 cites W2221984165 @default.
• W1985265215 cites W2492101850 @default.
• W1985265215 cites W2952548584 @default.
• W1985265215 cites W4211039187 @default.
• W1985265215 cites W4211216439 @default.
• W1985265215 cites W1996292687 @default.
• W1985265215 doi "https://doi.org/10.1021/ja803336y" @default.
• W1985265215 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2633102" @default.
• W1985265215 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18803382" @default.
• W1985265215 hasPublicationYear "2008" @default.
• W1985265215 type Work @default.
• W1985265215 sameAs 1985265215 @default.
• W1985265215 citedByCount "30" @default.
• W1985265215 countsByYear W19852652152012 @default.
• W1985265215 countsByYear W19852652152013 @default.
• W1985265215 countsByYear W19852652152014 @default.
• W1985265215 countsByYear W19852652152016 @default.
• W1985265215 countsByYear W19852652152018 @default.
• W1985265215 countsByYear W19852652152019 @default.
• W1985265215 countsByYear W19852652152020 @default.
• W1985265215 countsByYear W19852652152022 @default.
• W1985265215 countsByYear W19852652152023 @default.
• W1985265215 crossrefType "journal-article" @default.
• W1985265215 hasAuthorship W1985265215A5039902482 @default.
• W1985265215 hasAuthorship W1985265215A5066873256 @default.
• W1985265215 hasBestOaLocation W19852652152 @default.
• W1985265215 hasConcept C104317684 @default.
• W1985265215 hasConcept C112887158 @default.
• W1985265215 hasConcept C145148216 @default.
• W1985265215 hasConcept C178790620 @default.
• W1985265215 hasConcept C185581394 @default.
• W1985265215 hasConcept C185592680 @default.
• W1985265215 hasConcept C21951064 @default.
• W1985265215 hasConcept C2776543447 @default.
• W1985265215 hasConcept C2776991684 @default.
• W1985265215 hasConcept C30095370 @default.
• W1985265215 hasConcept C32909587 @default.
• W1985265215 hasConcept C512185932 @default.
• W1985265215 hasConcept C552990157 @default.
• W1985265215 hasConcept C55493867 @default.

• next