FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985274927> ?p ?o ?g. }

• W1985274927 endingPage "4784" @default.
• W1985274927 startingPage "4773" @default.
• W1985274927 abstract "Little is known about the function of flavivirus nonstructural protein NS2A. Two forms of NS2A are found in yellow fever virus-infected cells. Full-length NS2A (224 amino acids) is the product of cleavage at the NS1/2A and NS2A/2B sites. NS2Aalpha, a C-terminally truncated form of 190 amino acids, results from partial cleavage by the viral NS2B-3 serine protease at the sequence QK /T within NS2A. Exchange of serine for lysine at this site (QKT-->QST) blocks the production of both NS2Aalpha and infectious virus. The present study reveals that this defect is not at the level of RNA replication. Despite normal structural region processing, infectious particles containing genome RNA and capsid protein were not released from cells transfected with the mutant RNA. Nevertheless, production of subviral prM/M- and E-containing particles was unimpaired. The NS2A defect could be complemented in trans by providing NS1-2A or NS1-2Aalpha. However, trans complementation was not observed when the C-terminal lysine of NS1-2Aalpha was replaced with serine. In addition to true reversions, NS2Aalpha cleavage site mutations could be suppressed by two classes of second-site changes. The first class consisted of insertions at the NS2Aalpha cleavage site that restored its basic character and cleavability. A second class of suppressors occurred in the NS3 helicase domain, in which NS3 aspartate 343 was replaced with an uncharged residue (either valine, alanine, or glycine). These mutations in NS3 restored infectious-virus production in the absence of cleavage at the mutant NS2Aalpha site. Taken together, our results reveal an unexpected role for NS2A and NS3 in the assembly and/or release of infectious flavivirus particles." @default.
• W1985274927 created "2016-06-24" @default.
• W1985274927 creator A5019683467 @default.
• W1985274927 creator A5060109392 @default.
• W1985274927 date "2002-05-15" @default.
• W1985274927 modified "2023-10-02" @default.
• W1985274927 title "Mutations in the Yellow Fever Virus Nonstructural Protein NS2A Selectively Block Production of Infectious Particles" @default.
• W1985274927 cites W1487855743 @default.
• W1985274927 cites W1511043959 @default.
• W1985274927 cites W1524967393 @default.
• W1985274927 cites W1530508050 @default.
• W1985274927 cites W1569265439 @default.
• W1985274927 cites W1770656784 @default.
• W1985274927 cites W1851383444 @default.
• W1985274927 cites W1911781239 @default.
• W1985274927 cites W1912434761 @default.
• W1985274927 cites W1933943651 @default.
• W1985274927 cites W1967083901 @default.
• W1985274927 cites W1969673444 @default.
• W1985274927 cites W1973565185 @default.
• W1985274927 cites W1975085160 @default.
• W1985274927 cites W1978035722 @default.
• W1985274927 cites W1998936302 @default.
• W1985274927 cites W2015471688 @default.
• W1985274927 cites W2018289835 @default.
• W1985274927 cites W2030303541 @default.
• W1985274927 cites W2044289012 @default.
• W1985274927 cites W2057829396 @default.
• W1985274927 cites W2097382368 @default.
• W1985274927 cites W2100837269 @default.
• W1985274927 cites W2102976587 @default.
• W1985274927 cites W2103669924 @default.
• W1985274927 cites W2121374421 @default.
• W1985274927 cites W2125415345 @default.
• W1985274927 cites W2131331700 @default.
• W1985274927 cites W2142481964 @default.
• W1985274927 cites W2145130602 @default.
• W1985274927 cites W2161308145 @default.
• W1985274927 cites W2162464744 @default.
• W1985274927 cites W2163412375 @default.
• W1985274927 cites W4248600275 @default.
• W1985274927 doi "https://doi.org/10.1128/jvi.76.10.4773-4784.2002" @default.
• W1985274927 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/136122" @default.
• W1985274927 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11967294" @default.
• W1985274927 hasPublicationYear "2002" @default.
• W1985274927 type Work @default.
• W1985274927 sameAs 1985274927 @default.
• W1985274927 citedByCount "271" @default.
• W1985274927 countsByYear W19852749272012 @default.
• W1985274927 countsByYear W19852749272013 @default.
• W1985274927 countsByYear W19852749272014 @default.
• W1985274927 countsByYear W19852749272015 @default.
• W1985274927 countsByYear W19852749272016 @default.
• W1985274927 countsByYear W19852749272017 @default.
• W1985274927 countsByYear W19852749272018 @default.
• W1985274927 countsByYear W19852749272019 @default.
• W1985274927 countsByYear W19852749272020 @default.
• W1985274927 countsByYear W19852749272021 @default.
• W1985274927 countsByYear W19852749272022 @default.
• W1985274927 countsByYear W19852749272023 @default.
• W1985274927 crossrefType "journal-article" @default.
• W1985274927 hasAuthorship W1985274927A5019683467 @default.
• W1985274927 hasAuthorship W1985274927A5060109392 @default.
• W1985274927 hasBestOaLocation W19852749272 @default.
• W1985274927 hasConcept C104317684 @default.
• W1985274927 hasConcept C132379496 @default.
• W1985274927 hasConcept C143065580 @default.
• W1985274927 hasConcept C153911025 @default.
• W1985274927 hasConcept C159047783 @default.
• W1985274927 hasConcept C181199279 @default.
• W1985274927 hasConcept C188082640 @default.
• W1985274927 hasConcept C202878990 @default.
• W1985274927 hasConcept C2522874641 @default.
• W1985274927 hasConcept C2776408679 @default.
• W1985274927 hasConcept C2776414213 @default.
• W1985274927 hasConcept C2779856020 @default.
• W1985274927 hasConcept C2780568436 @default.
• W1985274927 hasConcept C515207424 @default.
• W1985274927 hasConcept C55493867 @default.
• W1985274927 hasConcept C67705224 @default.
• W1985274927 hasConcept C86803240 @default.
• W1985274927 hasConceptScore W1985274927C104317684 @default.
• W1985274927 hasConceptScore W1985274927C132379496 @default.
• W1985274927 hasConceptScore W1985274927C143065580 @default.
• W1985274927 hasConceptScore W1985274927C153911025 @default.
• W1985274927 hasConceptScore W1985274927C159047783 @default.
• W1985274927 hasConceptScore W1985274927C181199279 @default.
• W1985274927 hasConceptScore W1985274927C188082640 @default.
• W1985274927 hasConceptScore W1985274927C202878990 @default.
• W1985274927 hasConceptScore W1985274927C2522874641 @default.
• W1985274927 hasConceptScore W1985274927C2776408679 @default.
• W1985274927 hasConceptScore W1985274927C2776414213 @default.
• W1985274927 hasConceptScore W1985274927C2779856020 @default.
• W1985274927 hasConceptScore W1985274927C2780568436 @default.
• W1985274927 hasConceptScore W1985274927C515207424 @default.
• W1985274927 hasConceptScore W1985274927C55493867 @default.
• W1985274927 hasConceptScore W1985274927C67705224 @default.
• W1985274927 hasConceptScore W1985274927C86803240 @default.

• next