FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985280270> ?p ?o ?g. }

• W1985280270 endingPage "6264" @default.
• W1985280270 startingPage "6252" @default.
• W1985280270 abstract "The first kinetic study of a substrate (CN(-)) binding to the isolated active site (extracted FeMo-cofactor) of nitrogenase is described. The kinetics of the reactions between CN(-) and various derivatives of extracted FeMo-cofactor [FeMoco-L; where L is bound to Mo, and is NMF, Bu(t)NC, or imidazole (ImH)] have been followed using a stopped-flow, sequential-mix method in which the course of the reaction is followed indirectly, by monitoring the change in the rate of the reaction of the cofactor with PhS(-). The kinetic results, together with DFT calculations, indicate that the initial site of CN(-) binding to FeMoco-L is controlled by a combination of the electron-richness of the cluster core and lability of the Mo-L bond. Ultimately, the reactions between FeMoco-L and CN(-) involve displacement of L and binding of CN(-) to Mo. These reactions occur with a variety of rates and rate laws dependent on the nature of L. For FeMoco-NMF, the reaction with CN(-) is complete within the dead-time of the apparatus (ca. 4 ms), while with FeMoco-CNBu(t) the reaction is much slower and exhibits first order dependences on the concentrations of both FeMoco-CNBu(t) and CN(-) (k = 2.5 +/- 0.5 x 10(4) dm(3) mol(-1) s(-1)). The reaction of FeMoco-ImH with CN(-) occurs at a rate which exhibits a first order dependence on FeMoco-ImH but is independent of the concentration of CN(-) (k = 50 +/- 10 s(-1)). The results are interpreted in terms of CN(-) binding directly to the Mo site for FeMoco-NMF and FeMoco-ImH, but with FeMoco-CNBu(t) initial binding at an Fe site is followed by movement of CN(-) to Mo. Complementary DFT calculations are consistent with this interpretation, indicating that, in FeMoco-L, the Mo-L bond is stronger for L = ImH than for L = CNBu(t) and the binding of CN(-) to Mo is stronger than to any Fe atom in the cofactor." @default.
• W1985280270 created "2016-06-24" @default.
• W1985280270 creator A5002463554 @default.
• W1985280270 creator A5042933032 @default.
• W1985280270 creator A5073357045 @default.
• W1985280270 creator A5088664605 @default.
• W1985280270 creator A5089311673 @default.
• W1985280270 date "2003-09-03" @default.
• W1985280270 modified "2023-10-16" @default.
• W1985280270 title "Binding Sites of Nitrogenase: Kinetic and Theoretical Studies of Cyanide Binding to Extracted FeMo-Cofactor Derivatives" @default.
• W1985280270 cites W1551263566 @default.
• W1985280270 cites W1861570022 @default.
• W1985280270 cites W1927011268 @default.
• W1985280270 cites W1963677358 @default.
• W1985280270 cites W1963872103 @default.
• W1985280270 cites W1964798047 @default.
• W1985280270 cites W1972983175 @default.
• W1985280270 cites W1986546695 @default.
• W1985280270 cites W1987871569 @default.
• W1985280270 cites W2011994941 @default.
• W1985280270 cites W2016173440 @default.
• W1985280270 cites W2019904617 @default.
• W1985280270 cites W2024369036 @default.
• W1985280270 cites W2027331703 @default.
• W1985280270 cites W2030952699 @default.
• W1985280270 cites W2049756815 @default.
• W1985280270 cites W2051798700 @default.
• W1985280270 cites W2069005428 @default.
• W1985280270 cites W2074152783 @default.
• W1985280270 cites W2075169074 @default.
• W1985280270 cites W2078752527 @default.
• W1985280270 cites W2080478920 @default.
• W1985280270 cites W2085411109 @default.
• W1985280270 cites W2085985409 @default.
• W1985280270 cites W2089078053 @default.
• W1985280270 cites W2125084167 @default.
• W1985280270 cites W2950392507 @default.
• W1985280270 cites W3135943103 @default.
• W1985280270 doi "https://doi.org/10.1021/ic030108q" @default.
• W1985280270 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14514300" @default.
• W1985280270 hasPublicationYear "2003" @default.
• W1985280270 type Work @default.
• W1985280270 sameAs 1985280270 @default.
• W1985280270 citedByCount "18" @default.
• W1985280270 countsByYear W19852802702014 @default.
• W1985280270 countsByYear W19852802702020 @default.
• W1985280270 crossrefType "journal-article" @default.
• W1985280270 hasAuthorship W1985280270A5002463554 @default.
• W1985280270 hasAuthorship W1985280270A5042933032 @default.
• W1985280270 hasAuthorship W1985280270A5073357045 @default.
• W1985280270 hasAuthorship W1985280270A5088664605 @default.
• W1985280270 hasAuthorship W1985280270A5089311673 @default.
• W1985280270 hasConcept C107824862 @default.
• W1985280270 hasConcept C111368507 @default.
• W1985280270 hasConcept C127313418 @default.
• W1985280270 hasConcept C141280058 @default.
• W1985280270 hasConcept C161790260 @default.
• W1985280270 hasConcept C178790620 @default.
• W1985280270 hasConcept C181199279 @default.
• W1985280270 hasConcept C181440489 @default.
• W1985280270 hasConcept C185592680 @default.
• W1985280270 hasConcept C197957613 @default.
• W1985280270 hasConcept C2777289219 @default.
• W1985280270 hasConcept C41183919 @default.
• W1985280270 hasConcept C537208039 @default.
• W1985280270 hasConcept C55493867 @default.
• W1985280270 hasConcept C71240020 @default.
• W1985280270 hasConcept C8010536 @default.
• W1985280270 hasConceptScore W1985280270C107824862 @default.
• W1985280270 hasConceptScore W1985280270C111368507 @default.
• W1985280270 hasConceptScore W1985280270C127313418 @default.
• W1985280270 hasConceptScore W1985280270C141280058 @default.
• W1985280270 hasConceptScore W1985280270C161790260 @default.
• W1985280270 hasConceptScore W1985280270C178790620 @default.
• W1985280270 hasConceptScore W1985280270C181199279 @default.
• W1985280270 hasConceptScore W1985280270C181440489 @default.
• W1985280270 hasConceptScore W1985280270C185592680 @default.
• W1985280270 hasConceptScore W1985280270C197957613 @default.
• W1985280270 hasConceptScore W1985280270C2777289219 @default.
• W1985280270 hasConceptScore W1985280270C41183919 @default.
• W1985280270 hasConceptScore W1985280270C537208039 @default.
• W1985280270 hasConceptScore W1985280270C55493867 @default.
• W1985280270 hasConceptScore W1985280270C71240020 @default.
• W1985280270 hasConceptScore W1985280270C8010536 @default.
• W1985280270 hasIssue "20" @default.
• W1985280270 hasLocation W19852802701 @default.
• W1985280270 hasLocation W19852802702 @default.
• W1985280270 hasOpenAccess W1985280270 @default.
• W1985280270 hasPrimaryLocation W19852802701 @default.
• W1985280270 hasRelatedWork W1977907338 @default.
• W1985280270 hasRelatedWork W2007545289 @default.
• W1985280270 hasRelatedWork W2158401745 @default.
• W1985280270 hasRelatedWork W2210062196 @default.
• W1985280270 hasRelatedWork W2313870002 @default.
• W1985280270 hasRelatedWork W2979863795 @default.
• W1985280270 hasRelatedWork W2982904704 @default.
• W1985280270 hasRelatedWork W3112607042 @default.
• W1985280270 hasRelatedWork W3186257491 @default.

• next