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- W1985291272 abstract "Aqueous dispersions of bicelles (bilayered micelles) offer an alternative to mechanically aligned bilayer samples for solid-state NMR spectroscopy (Sanders and Landis, 1995, Biochemistry 43:4030-4040). Bicelle formation requires at least a binary mixture of zwitterionic long-chain and short-chain lipids. The long lipid (such as 1,2-dimyristoyl-sn-glycero-3-phosphocholine) forms a planar bilayer surface, while the short-chain lipid (such as 1,2-di-O-hexyl-sn-glycero-3-phosphocholine), caps the edges and isolates the bilayer hydrophobic core from water. When placed in a magnetic field, bicelles may spontaneously align. Because bacterial cell membranes are composed of 20%-25% negatively-charged lipids, we have sought to include an anionic lipid (such as 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol) in our bicelle preparations. To find optimal conditions, we have varied the content of the negatively charged lipid, the ratio of long to short-chain lipid (q), and the buffer used to prepare the bicelles. 31P NMR spectroscopy was used to assess the alignment of the bicelles under the various conditions. Membrane active peptides, selectively deuterated at specific amino acids, will be incorporated into bicelles under optimal conditions and into mechanically aligned bilayers on glass plates. NMR spectra from membrane active peptides in mixed lipid bicelles will be compared with spectra from peptides in macroscopically oriented bilayers prepared on glass slides. Studies of cationic antimicrobial peptides, such as lactoferricin B6 (RRWQWR-NH2) are significant because of the increased emergence of antibiotic resistant bacterial strains. It is therefore important to understand the mechanism by which such peptides exert effects on membranes which mimic the composition of bacterial cell membranes." @default.
- W1985291272 created "2016-06-24" @default.
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- W1985291272 date "2010-01-01" @default.
- W1985291272 modified "2023-10-16" @default.
- W1985291272 title "Development of Bicelles Containing Anionic Lipids to Characterize Cationic Membrane Active Peptides by NMR Spectroscopy" @default.
- W1985291272 doi "https://doi.org/10.1016/j.bpj.2009.12.517" @default.
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