FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985293825> ?p ?o ?g. }

• W1985293825 endingPage "101" @default.
• W1985293825 startingPage "59" @default.
• W1985293825 abstract "The in vitro alkaline elution/rat hepatocyte assay is a sensitive assay for genotoxicity, measured as DNA strand breaks induced in primary cultures of rat hepatocytes after 3-h treatments with test compounds. Since DNA degradation can be rapid and extensive in dead and/or dying cells, the original criteria for a positive result in the assay were that a compound induce a 3.0-fold or greater increase in the elution slope (for the terminal phase of alkaline elution from 3 to 9 h) in the absence of significant cytotoxicity (defined as relative cell viability of less than 70% by trypan blue dye exclusion; TBDE). Recently we have shown that false-positive results can still be obtained due to cytotoxicity when loss of membrane integrity is a late event in toxic cell death relative to the induction of endonucleolytic DNA degradation. To improve the ability of the assay to discriminate between genotoxic vs. cytotoxic effects of chemicals, we have evaluated additional assays of cytotoxicity including cell adenosine triphosphate (ATP) and potassium (K +) content, tetrazolium dye reduction (MTT), TBDE after a further 3-h recovery incubation without test chemicals (delayed toxicity), cell blebbing and endonucleolytic DNA degradation (double-strand breaks; DSBs) assessed by pulsed-field gel electrophoresis (PFGE). We have also evaluated 2 parameters derived from the elution data which can indicate extensive, eytotoxicity-induced DNA degradation: the fraction of the DNA recovered in the neutral lysis/rinse fraction and the v-intercept of the extrapolation of the 3-9-h segment of the elution curve. Twenty-eight rodent non-carcinogens that are negative (or inconclusive) in the Ames assay with no, or limited, other evidence of genotoxicity, and 33 genotoxins, most of which are also carcinogens, were evaluated. The results showed that DNA degradation as measured by a 1-h PACE (Programmed Autonomously Controlled Electrodes)/PFGE assay was a sensitive indicator of cytotoxicity which correlated well with results of the other cytotoxicity indicators. The delayed TBDE (after a 3-h recovery), intracellular potassium and ATP assays as well as the v-intercept parameter were also shown to be sensitive and in some cases complementary measures of cytotoxicity. Using new criteria based on these data of an induced slope (treatment slope-negative control slope) of 0.020 for the 3- to 9-h elution period and cytotoxicity limits of 70% relative viability for the delayed TBDE assay and 50% for intracellular ATP content, the assay scores the genotoxicity of these 61 reference compounds with an overall accuracy of 92%. Test results using these new criteria are provided for an additional 20 compounds (5 non-genotoxic carcinogens and 15 compounds whose genotoxic and carcinogenic potential are unknown or equivocal)." @default.
• W1985293825 created "2016-06-24" @default.
• W1985293825 creator A5005835391 @default.
• W1985293825 creator A5016891242 @default.
• W1985293825 creator A5020423055 @default.
• W1985293825 creator A5035745217 @default.
• W1985293825 creator A5055429671 @default.
• W1985293825 creator A5057039180 @default.
• W1985293825 creator A5057644991 @default.
• W1985293825 creator A5067307545 @default.
• W1985293825 date "1996-06-01" @default.
• W1985293825 modified "2023-09-23" @default.
• W1985293825 title "Revalidation of the in vitro alkaline elution/rat hepatocyte assay for DNA damage: improved criteria for assessment of cytotoxicity and genotoxicity and results for 81 compounds" @default.
• W1985293825 cites W1543673064 @default.
• W1985293825 cites W1595366583 @default.
• W1985293825 cites W1968426926 @default.
• W1985293825 cites W1968541657 @default.
• W1985293825 cites W1971730609 @default.
• W1985293825 cites W1986226378 @default.
• W1985293825 cites W1986449016 @default.
• W1985293825 cites W1990896120 @default.
• W1985293825 cites W1998285677 @default.
• W1985293825 cites W2007350178 @default.
• W1985293825 cites W2007829362 @default.
• W1985293825 cites W2023474297 @default.
• W1985293825 cites W2029130914 @default.
• W1985293825 cites W2033885833 @default.
• W1985293825 cites W2045947881 @default.
• W1985293825 cites W2048142276 @default.
• W1985293825 cites W2050454410 @default.
• W1985293825 cites W2052063426 @default.
• W1985293825 cites W2052178794 @default.
• W1985293825 cites W2066640026 @default.
• W1985293825 cites W2068858653 @default.
• W1985293825 cites W2078150483 @default.
• W1985293825 cites W2078581194 @default.
• W1985293825 cites W2079461415 @default.
• W1985293825 cites W2083336181 @default.
• W1985293825 cites W2094875326 @default.
• W1985293825 cites W2099647529 @default.
• W1985293825 cites W2100818953 @default.
• W1985293825 cites W2108355409 @default.
• W1985293825 cites W2155174050 @default.
• W1985293825 cites W2261282430 @default.
• W1985293825 doi "https://doi.org/10.1016/0165-1218(95)00070-4" @default.
• W1985293825 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8684406" @default.
• W1985293825 hasPublicationYear "1996" @default.
• W1985293825 type Work @default.
• W1985293825 sameAs 1985293825 @default.
• W1985293825 citedByCount "131" @default.
• W1985293825 countsByYear W19852938252012 @default.
• W1985293825 countsByYear W19852938252013 @default.
• W1985293825 countsByYear W19852938252014 @default.
• W1985293825 countsByYear W19852938252015 @default.
• W1985293825 countsByYear W19852938252016 @default.
• W1985293825 countsByYear W19852938252017 @default.
• W1985293825 countsByYear W19852938252018 @default.
• W1985293825 countsByYear W19852938252019 @default.
• W1985293825 countsByYear W19852938252020 @default.
• W1985293825 countsByYear W19852938252022 @default.
• W1985293825 countsByYear W19852938252023 @default.
• W1985293825 crossrefType "journal-article" @default.
• W1985293825 hasAuthorship W1985293825A5005835391 @default.
• W1985293825 hasAuthorship W1985293825A5016891242 @default.
• W1985293825 hasAuthorship W1985293825A5020423055 @default.
• W1985293825 hasAuthorship W1985293825A5035745217 @default.
• W1985293825 hasAuthorship W1985293825A5055429671 @default.
• W1985293825 hasAuthorship W1985293825A5057039180 @default.
• W1985293825 hasAuthorship W1985293825A5057644991 @default.
• W1985293825 hasAuthorship W1985293825A5067307545 @default.
• W1985293825 hasConcept C109316439 @default.
• W1985293825 hasConcept C116084860 @default.
• W1985293825 hasConcept C143425029 @default.
• W1985293825 hasConcept C153911025 @default.
• W1985293825 hasConcept C178790620 @default.
• W1985293825 hasConcept C185592680 @default.
• W1985293825 hasConcept C202751555 @default.
• W1985293825 hasConcept C2778959862 @default.
• W1985293825 hasConcept C29730261 @default.
• W1985293825 hasConcept C43617362 @default.
• W1985293825 hasConcept C552990157 @default.
• W1985293825 hasConcept C55493867 @default.
• W1985293825 hasConcept C57409179 @default.
• W1985293825 hasConcept C86803240 @default.
• W1985293825 hasConceptScore W1985293825C109316439 @default.
• W1985293825 hasConceptScore W1985293825C116084860 @default.
• W1985293825 hasConceptScore W1985293825C143425029 @default.
• W1985293825 hasConceptScore W1985293825C153911025 @default.
• W1985293825 hasConceptScore W1985293825C178790620 @default.
• W1985293825 hasConceptScore W1985293825C185592680 @default.
• W1985293825 hasConceptScore W1985293825C202751555 @default.
• W1985293825 hasConceptScore W1985293825C2778959862 @default.
• W1985293825 hasConceptScore W1985293825C29730261 @default.
• W1985293825 hasConceptScore W1985293825C43617362 @default.
• W1985293825 hasConceptScore W1985293825C552990157 @default.
• W1985293825 hasConceptScore W1985293825C55493867 @default.
• W1985293825 hasConceptScore W1985293825C57409179 @default.
• W1985293825 hasConceptScore W1985293825C86803240 @default.

• next