Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985353668> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1985353668 endingPage "1126" @default.
- W1985353668 startingPage "1122" @default.
- W1985353668 abstract "Human apolipoprotein A-1 was formulated in Immune Stimulating Complexes (ISCOMs). The structure of the protein in ISCOMs was examined directly using several biophysical techniques including Fourier transform infrared (FTIR) spectroscopy, near UV circular dichroism (CD), and fluorescence spectroscopy. Amide I FTIR data indicate that human apolipoprotein A-1 displays a slightly increased alpha-helical content after its incorporation into ISCOMs. Near UV CD and tryptophan fluorescence data suggest that association with ISCOMs results in the tryptophan residues of the protein experiencing a relatively hydrophobic environment, motional restriction, and local electrostatic interactions. These observations are consistent with an increased order in the protein structure upon incorporation in ISCOMs. In addition, biomolecular interaction analysis (BIA), based on surface plasmon resonance (SPR) measurements, suggests that the binding affinity of human apolipoprotein A-1 to a monoclonal anti-human apolipoprotein A-1 antibody is moderately decreased (by 20%) after its incorporation into ISCOMs. This study demonstrates that these biophysical techniques can be used to noninvasively monitor integrity of or changes in secondary and tertiary structure of proteins within the ISCOM particles without the need for protein extraction." @default.
- W1985353668 created "2016-06-24" @default.
- W1985353668 creator A5014345880 @default.
- W1985353668 creator A5063816637 @default.
- W1985353668 date "1999-11-01" @default.
- W1985353668 modified "2023-09-23" @default.
- W1985353668 title "Direct biophysical characterization of human apolipoprotein A-1 in ISCOMs" @default.
- W1985353668 cites W1013500213 @default.
- W1985353668 cites W1632470944 @default.
- W1985353668 cites W2007242412 @default.
- W1985353668 cites W2014945371 @default.
- W1985353668 cites W2025372248 @default.
- W1985353668 cites W2040713377 @default.
- W1985353668 cites W2069520354 @default.
- W1985353668 cites W2106498165 @default.
- W1985353668 cites W2161267502 @default.
- W1985353668 cites W2181164354 @default.
- W1985353668 cites W2209250776 @default.
- W1985353668 cites W2212272536 @default.
- W1985353668 doi "https://doi.org/10.1021/js990158l" @default.
- W1985353668 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10564059" @default.
- W1985353668 hasPublicationYear "1999" @default.
- W1985353668 type Work @default.
- W1985353668 sameAs 1985353668 @default.
- W1985353668 citedByCount "6" @default.
- W1985353668 countsByYear W19853536682012 @default.
- W1985353668 countsByYear W19853536682017 @default.
- W1985353668 crossrefType "journal-article" @default.
- W1985353668 hasAuthorship W1985353668A5014345880 @default.
- W1985353668 hasAuthorship W1985353668A5063816637 @default.
- W1985353668 hasConcept C106847996 @default.
- W1985353668 hasConcept C12554922 @default.
- W1985353668 hasConcept C127413603 @default.
- W1985353668 hasConcept C133571119 @default.
- W1985353668 hasConcept C155672457 @default.
- W1985353668 hasConcept C160892712 @default.
- W1985353668 hasConcept C171250308 @default.
- W1985353668 hasConcept C185592680 @default.
- W1985353668 hasConcept C192562407 @default.
- W1985353668 hasConcept C2776706248 @default.
- W1985353668 hasConcept C2778163477 @default.
- W1985353668 hasConcept C42360764 @default.
- W1985353668 hasConcept C47701112 @default.
- W1985353668 hasConcept C515207424 @default.
- W1985353668 hasConcept C55493867 @default.
- W1985353668 hasConcept C62614982 @default.
- W1985353668 hasConcept C62746215 @default.
- W1985353668 hasConcept C75599170 @default.
- W1985353668 hasConcept C8010536 @default.
- W1985353668 hasConcept C86803240 @default.
- W1985353668 hasConceptScore W1985353668C106847996 @default.
- W1985353668 hasConceptScore W1985353668C12554922 @default.
- W1985353668 hasConceptScore W1985353668C127413603 @default.
- W1985353668 hasConceptScore W1985353668C133571119 @default.
- W1985353668 hasConceptScore W1985353668C155672457 @default.
- W1985353668 hasConceptScore W1985353668C160892712 @default.
- W1985353668 hasConceptScore W1985353668C171250308 @default.
- W1985353668 hasConceptScore W1985353668C185592680 @default.
- W1985353668 hasConceptScore W1985353668C192562407 @default.
- W1985353668 hasConceptScore W1985353668C2776706248 @default.
- W1985353668 hasConceptScore W1985353668C2778163477 @default.
- W1985353668 hasConceptScore W1985353668C42360764 @default.
- W1985353668 hasConceptScore W1985353668C47701112 @default.
- W1985353668 hasConceptScore W1985353668C515207424 @default.
- W1985353668 hasConceptScore W1985353668C55493867 @default.
- W1985353668 hasConceptScore W1985353668C62614982 @default.
- W1985353668 hasConceptScore W1985353668C62746215 @default.
- W1985353668 hasConceptScore W1985353668C75599170 @default.
- W1985353668 hasConceptScore W1985353668C8010536 @default.
- W1985353668 hasConceptScore W1985353668C86803240 @default.
- W1985353668 hasIssue "11" @default.
- W1985353668 hasLocation W19853536681 @default.
- W1985353668 hasLocation W19853536682 @default.
- W1985353668 hasOpenAccess W1985353668 @default.
- W1985353668 hasPrimaryLocation W19853536681 @default.
- W1985353668 hasRelatedWork W1978592925 @default.
- W1985353668 hasRelatedWork W1985353668 @default.
- W1985353668 hasRelatedWork W2020577335 @default.
- W1985353668 hasRelatedWork W2050461444 @default.
- W1985353668 hasRelatedWork W2059363535 @default.
- W1985353668 hasRelatedWork W2065546252 @default.
- W1985353668 hasRelatedWork W2117865231 @default.
- W1985353668 hasRelatedWork W2330980444 @default.
- W1985353668 hasRelatedWork W2421235400 @default.
- W1985353668 hasRelatedWork W2933325505 @default.
- W1985353668 hasVolume "88" @default.
- W1985353668 isParatext "false" @default.
- W1985353668 isRetracted "false" @default.
- W1985353668 magId "1985353668" @default.
- W1985353668 workType "article" @default.