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- W1985359084 abstract "In a previous study sera from children with cow's milk protein intolerance (CMPI) exhibiting gastrointestinal symptoms were found to efficiently induce antibody-dependent cell-mediated cyto-toxicity (ADCC) to β-lactoglobulin-coated cells. In contrast, sera from children with coeliac disease showed a low ADCC-mediating capacity, despite high levels of IgG anti-β-lactoglobulin antibodies. The study described here was undertaken to evaluate whether differences in IgG subclass profile of anti-β-lactoglobulin antibodies could explain the observed variations in the ADCC-mediating capacity. Forty-eight sera from the following groups of children were investigated: CMPI with predominantly gastrointestinal symptoms, CMPI with skin symptoms of immediate-onset, children with untreated coeliac disease and healthy references. Absorption experiments indicated that primarily IgGl antibodies were responsible for the ADCC-mediating capacity of the sera. Accordingly, the ADCC reactivity of individual sera correlated with their IgGl antibody levels. Sera from CMPI children with gastrointes tinal symptoms, most of which had a high ADCC reactivity, also demonstrated a distinctive subclass pattern of their anti-β-lactoglobulin antibodies with higher relative proportions of IgGl (ratios: IgGl/IgG, IgGl/IgG3 and IgGl/IgG4) than those from the other diagnostic groups. Using logistic regression analysis, the diagnostic potential of ADCC as well as of different IgG subclass variables for the recognition of gastrointestinal symptoms caused by CMPI was evaluated. The ADCC reactivity of sera was found to be the best predictor in this model." @default.
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- W1985359084 date "1995-07-01" @default.
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- W1985359084 title "ADCC-Mediating Capacity in Children with Cow's Milk Protein Intolerance in Relation to IgG Subclass Profile of Serum Antibodies to beta-Lactoglobulin" @default.
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- W1985359084 doi "https://doi.org/10.1111/j.1365-3083.1995.tb03637.x" @default.
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