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• W1985374017 abstract "The dual-vector system-II (DVS-II), which allows efficient display of Fab antibodies on phage, has been reported previously, but its practical applicability in a phage-displayed antibody library has not been verified. To resolve this issue, we created two small combinatorial human Fab antibody libraries using the DVS-II, and isolation of target-specific antibodies was attempted. Biopanning of one antibody library, termed DVFAB-1L library, which has a 1.3 × 107 combinatorial antibody complexity, against fluorescein-BSA resulted in successful isolation of human Fab clones specific for the antigen despite the presence of only a single light chain in the library. By using the unique feature of the DVS-II, an antibody library of a larger size, named DVFAB-131L, which has a 1.5 × 109 combinatorial antibody complexity, was also generated in a rapid manner by combining 1.3 × 107 heavy chains and 131 light chains and more diverse anti-fluorescein-BSA Fab antibody clones were successfully obtained. Our results demonstrate that the DVS-II can be applied readily in creating phage-displayed antibody libraries with much less effort, and target-specific antibody clones can be isolated reliably via light chain promiscuity of antibody molecule" @default.
• W1985374017 created "2016-06-24" @default.
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• W1985374017 date "2009-03-01" @default.
• W1985374017 modified "2023-09-26" @default.
• W1985374017 title "Successful application of the dual-vector system II in creating a reliable phage-displayed combinatorial Fab library" @default.
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• W1985374017 doi "https://doi.org/10.1007/s10059-009-0040-0" @default.
• W1985374017 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19326078" @default.
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