Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985383014> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W1985383014 endingPage "364" @default.
- W1985383014 startingPage "360" @default.
- W1985383014 abstract "The generation of molecular sensors based on peptide-displaying enzymes for the detection of antibodies or antigens represents an innovative field of protein engineering. The knowledge of the underlying molecular mechanisms of enzymatic modulation in such sensors would be of great importance for the rational construction and improvement of responsiveness of new peptide-enzyme molecules. Here we analyze the enzymatic characteristics of three different kinds of sensors based in engineered beta-galactosidase, alkaline phosphatase and beta-lactamase, to explore a common activation basis. We describe two different categories of enzyme sensors. In one of them, including only some modified beta-lactamases, the enzymatic activity is inhibited upon ligand binding and it seems to be caused by the steric coverage of the active site by the bound antibody. In a second group, embracing members of the three studied enzymes, the ability to be modulated upon effector binding depends on the ratio between the k(cat) of the engineered enzyme and the k(cat) of the intact enzyme. This proves a common mechanism for enzymatic modulation of enzyme biosensors that is probably caused by conformational effects induced by the bound antibody on the enzyme." @default.
- W1985383014 created "2016-06-24" @default.
- W1985383014 creator A5004506933 @default.
- W1985383014 creator A5042249122 @default.
- W1985383014 creator A5057224975 @default.
- W1985383014 date "2000-08-01" @default.
- W1985383014 modified "2023-10-09" @default.
- W1985383014 title "Molecular Mechanisms for Antibody-Mediated Modulation of Peptide-Displaying Enzyme Sensors" @default.
- W1985383014 cites W1705207625 @default.
- W1985383014 cites W1976371096 @default.
- W1985383014 cites W1976981370 @default.
- W1985383014 cites W1996514181 @default.
- W1985383014 cites W1997079623 @default.
- W1985383014 cites W2014608843 @default.
- W1985383014 cites W2021918499 @default.
- W1985383014 cites W2028208950 @default.
- W1985383014 cites W2035609763 @default.
- W1985383014 cites W2041736701 @default.
- W1985383014 cites W2041774877 @default.
- W1985383014 cites W2048617815 @default.
- W1985383014 cites W2085244603 @default.
- W1985383014 cites W2085945526 @default.
- W1985383014 cites W2166519851 @default.
- W1985383014 doi "https://doi.org/10.1006/bbrc.2000.3309" @default.
- W1985383014 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10964671" @default.
- W1985383014 hasPublicationYear "2000" @default.
- W1985383014 type Work @default.
- W1985383014 sameAs 1985383014 @default.
- W1985383014 citedByCount "22" @default.
- W1985383014 countsByYear W19853830142019 @default.
- W1985383014 countsByYear W19853830142022 @default.
- W1985383014 crossrefType "journal-article" @default.
- W1985383014 hasAuthorship W1985383014A5004506933 @default.
- W1985383014 hasAuthorship W1985383014A5042249122 @default.
- W1985383014 hasAuthorship W1985383014A5057224975 @default.
- W1985383014 hasConcept C147816474 @default.
- W1985383014 hasConcept C159654299 @default.
- W1985383014 hasConcept C160160445 @default.
- W1985383014 hasConcept C160756335 @default.
- W1985383014 hasConcept C178666793 @default.
- W1985383014 hasConcept C181199279 @default.
- W1985383014 hasConcept C185592680 @default.
- W1985383014 hasConcept C201194858 @default.
- W1985383014 hasConcept C2779281246 @default.
- W1985383014 hasConcept C41183919 @default.
- W1985383014 hasConcept C51785407 @default.
- W1985383014 hasConcept C53105671 @default.
- W1985383014 hasConcept C54355233 @default.
- W1985383014 hasConcept C55493867 @default.
- W1985383014 hasConcept C71240020 @default.
- W1985383014 hasConcept C86803240 @default.
- W1985383014 hasConceptScore W1985383014C147816474 @default.
- W1985383014 hasConceptScore W1985383014C159654299 @default.
- W1985383014 hasConceptScore W1985383014C160160445 @default.
- W1985383014 hasConceptScore W1985383014C160756335 @default.
- W1985383014 hasConceptScore W1985383014C178666793 @default.
- W1985383014 hasConceptScore W1985383014C181199279 @default.
- W1985383014 hasConceptScore W1985383014C185592680 @default.
- W1985383014 hasConceptScore W1985383014C201194858 @default.
- W1985383014 hasConceptScore W1985383014C2779281246 @default.
- W1985383014 hasConceptScore W1985383014C41183919 @default.
- W1985383014 hasConceptScore W1985383014C51785407 @default.
- W1985383014 hasConceptScore W1985383014C53105671 @default.
- W1985383014 hasConceptScore W1985383014C54355233 @default.
- W1985383014 hasConceptScore W1985383014C55493867 @default.
- W1985383014 hasConceptScore W1985383014C71240020 @default.
- W1985383014 hasConceptScore W1985383014C86803240 @default.
- W1985383014 hasIssue "2" @default.
- W1985383014 hasLocation W19853830141 @default.
- W1985383014 hasLocation W19853830142 @default.
- W1985383014 hasOpenAccess W1985383014 @default.
- W1985383014 hasPrimaryLocation W19853830141 @default.
- W1985383014 hasRelatedWork W1968905431 @default.
- W1985383014 hasRelatedWork W2024765299 @default.
- W1985383014 hasRelatedWork W2042181696 @default.
- W1985383014 hasRelatedWork W2554214300 @default.
- W1985383014 hasRelatedWork W2626793756 @default.
- W1985383014 hasRelatedWork W2749706380 @default.
- W1985383014 hasRelatedWork W3092454462 @default.
- W1985383014 hasRelatedWork W3215076145 @default.
- W1985383014 hasRelatedWork W4313643099 @default.
- W1985383014 hasRelatedWork W3082964343 @default.
- W1985383014 hasVolume "275" @default.
- W1985383014 isParatext "false" @default.
- W1985383014 isRetracted "false" @default.
- W1985383014 magId "1985383014" @default.
- W1985383014 workType "article" @default.