Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985397009> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1985397009 endingPage "5290" @default.
- W1985397009 startingPage "5286" @default.
- W1985397009 abstract "A photoaffinity labeling method was used to characterize and compare cyclic nucleotide-binding proteins of bovine liver cytosol with binding proteins of the nucleus. After photoaffinity labeling of cytosol with 8-azido cyclic [(32)P]AMP, autoradiographs of sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed two major labeled proteins of 47,000 and 52,000-55,000 daltons. DEAE-cellulose column-derived fractions suggested that the larger protein was the regulatory subunit of peak II cyclic AMP-dependent protein kinase and the smaller protein was the regulatory subunit of peak I kinase. The smaller protein was largely present as the free regulatory subunit. The two binding proteins differed in their ability to bind cyclic GMP. Binding to both proteins was abolished by excess unlabeled cyclic AMP but not by 5'-AMP. Photoaffinity labeling of a 0.14 M salt extract of nuclei and a nonhistone chromosomal protein preparation revealed two major binding proteins with the same molecular weight and competition profiles as those of the cytosol. Detergent-washed nuclei gave similar results. Several minor binding proteins were observed in both cytosol and nucleus. One protein (36,000 daltons) was unique to the nucleus and had low affinity for 8-azido cyclic AMP. Photoaffinity labeling with cyclic [(3)H]GMP revealed a cytosol protein, absent from the nucleus, of 31,000 daltons and the ligand was competed for by both cyclic GMP and 5'-GMP. These studies suggest that the major specific cyclic AMP-binding proteins of bovine liver are the type I and type II regulatory subunits of cyclic AMP-dependent protein kinase and are present in both nucleus and cytoplasm." @default.
- W1985397009 created "2016-06-24" @default.
- W1985397009 creator A5047938580 @default.
- W1985397009 creator A5088108188 @default.
- W1985397009 date "1978-11-01" @default.
- W1985397009 modified "2023-09-25" @default.
- W1985397009 title "Cyclic nucleotide-binding proteins detected by photoaffinity labeling in nucleus and cytoplasm of bovine liver" @default.
- W1985397009 cites W1494318461 @default.
- W1985397009 cites W1510725443 @default.
- W1985397009 cites W1558844889 @default.
- W1985397009 cites W1585983580 @default.
- W1985397009 cites W1620075378 @default.
- W1985397009 cites W1987804164 @default.
- W1985397009 cites W1996466550 @default.
- W1985397009 cites W2014583300 @default.
- W1985397009 cites W2018289835 @default.
- W1985397009 cites W2018491753 @default.
- W1985397009 cites W2029225257 @default.
- W1985397009 cites W2032522194 @default.
- W1985397009 cites W2059401445 @default.
- W1985397009 cites W2068366808 @default.
- W1985397009 cites W2073157727 @default.
- W1985397009 cites W2091240756 @default.
- W1985397009 cites W2100837269 @default.
- W1985397009 cites W2120331014 @default.
- W1985397009 cites W2233299729 @default.
- W1985397009 cites W2398022854 @default.
- W1985397009 cites W2402006860 @default.
- W1985397009 cites W2409259792 @default.
- W1985397009 doi "https://doi.org/10.1073/pnas.75.11.5286" @default.
- W1985397009 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/392947" @default.
- W1985397009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/214781" @default.
- W1985397009 hasPublicationYear "1978" @default.
- W1985397009 type Work @default.
- W1985397009 sameAs 1985397009 @default.
- W1985397009 citedByCount "17" @default.
- W1985397009 countsByYear W19853970092023 @default.
- W1985397009 crossrefType "journal-article" @default.
- W1985397009 hasAuthorship W1985397009A5047938580 @default.
- W1985397009 hasAuthorship W1985397009A5088108188 @default.
- W1985397009 hasBestOaLocation W19853970092 @default.
- W1985397009 hasConcept C104292427 @default.
- W1985397009 hasConcept C104317684 @default.
- W1985397009 hasConcept C107824862 @default.
- W1985397009 hasConcept C116084860 @default.
- W1985397009 hasConcept C181199279 @default.
- W1985397009 hasConcept C184235292 @default.
- W1985397009 hasConcept C185592680 @default.
- W1985397009 hasConcept C190062978 @default.
- W1985397009 hasConcept C2778524494 @default.
- W1985397009 hasConcept C2778581200 @default.
- W1985397009 hasConcept C512185932 @default.
- W1985397009 hasConcept C553089730 @default.
- W1985397009 hasConcept C55493867 @default.
- W1985397009 hasConcept C86339819 @default.
- W1985397009 hasConcept C86803240 @default.
- W1985397009 hasConcept C94966510 @default.
- W1985397009 hasConcept C97029542 @default.
- W1985397009 hasConcept C98539663 @default.
- W1985397009 hasConceptScore W1985397009C104292427 @default.
- W1985397009 hasConceptScore W1985397009C104317684 @default.
- W1985397009 hasConceptScore W1985397009C107824862 @default.
- W1985397009 hasConceptScore W1985397009C116084860 @default.
- W1985397009 hasConceptScore W1985397009C181199279 @default.
- W1985397009 hasConceptScore W1985397009C184235292 @default.
- W1985397009 hasConceptScore W1985397009C185592680 @default.
- W1985397009 hasConceptScore W1985397009C190062978 @default.
- W1985397009 hasConceptScore W1985397009C2778524494 @default.
- W1985397009 hasConceptScore W1985397009C2778581200 @default.
- W1985397009 hasConceptScore W1985397009C512185932 @default.
- W1985397009 hasConceptScore W1985397009C553089730 @default.
- W1985397009 hasConceptScore W1985397009C55493867 @default.
- W1985397009 hasConceptScore W1985397009C86339819 @default.
- W1985397009 hasConceptScore W1985397009C86803240 @default.
- W1985397009 hasConceptScore W1985397009C94966510 @default.
- W1985397009 hasConceptScore W1985397009C97029542 @default.
- W1985397009 hasConceptScore W1985397009C98539663 @default.
- W1985397009 hasIssue "11" @default.
- W1985397009 hasLocation W19853970091 @default.
- W1985397009 hasLocation W19853970092 @default.
- W1985397009 hasLocation W19853970093 @default.
- W1985397009 hasOpenAccess W1985397009 @default.
- W1985397009 hasPrimaryLocation W19853970091 @default.
- W1985397009 hasRelatedWork W1451751071 @default.
- W1985397009 hasRelatedWork W1643792176 @default.
- W1985397009 hasRelatedWork W1985397009 @default.
- W1985397009 hasRelatedWork W2027768367 @default.
- W1985397009 hasRelatedWork W2035261138 @default.
- W1985397009 hasRelatedWork W2062702516 @default.
- W1985397009 hasRelatedWork W2072522400 @default.
- W1985397009 hasRelatedWork W2170449409 @default.
- W1985397009 hasRelatedWork W2414021185 @default.
- W1985397009 hasRelatedWork W2422066138 @default.
- W1985397009 hasVolume "75" @default.
- W1985397009 isParatext "false" @default.
- W1985397009 isRetracted "false" @default.
- W1985397009 magId "1985397009" @default.
- W1985397009 workType "article" @default.