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- W1985429462 abstract "This report presents a simple procedure for staining 1-2 microns epoxy plastic sections of cells and mineralizing matrix present in fetal bovine bone tissue cultures. A 0.3% aqueous toluidine blue O solution was used as a cellular stain and was followed with 2% alizarin red S for the detection of calcium at sites of mineralization. Effects of concentration and pH of alizarin red S on the penetration of epon embedded thick sections were investigated. Optimal staining was achieved with a 2% aqueous alizarin red S solution adjusted to a pH of 5.5-6.5. This staining procedure provides unusually clear contrast between mineral and bone cells in plastic sections for light microscopy." @default.
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- W1985429462 date "1986-01-01" @default.
- W1985429462 modified "2023-09-27" @default.
- W1985429462 title "A Simple Method Using Alizarin Red S For the Detection of Calcium in Epoxy Resin Embedded Tissue" @default.
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- W1985429462 doi "https://doi.org/10.3109/10520298609110714" @default.
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