FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985440166> ?p ?o ?g. }

• W1985440166 endingPage "4807" @default.
• W1985440166 startingPage "4797" @default.
• W1985440166 abstract "The structures of inactive mutants D144A and E78Q of the glycoside hydrolase family 8 (GH-8) endo-beta-1,4-d-xylanase (pXyl) from the Antarctic bacterium Pseudoalteromonas haloplanktis TAH3a in complex with its substrate xylopentaose (at 1.95 A resolution) and product xylotriose (at 1.9 A resolution) have been determined by X-ray crystallography. A detailed comparative analysis of these with the apo-enzyme and with other GH-8 structures indicates an induced fit mechanism upon ligand binding whereby a number of conformational changes and, in particular, a repositioning of the proton donor into a more catalytically competent position occurs. This has also allowed for the description of protein-ligand interactions in this enzyme and for the demarcation of subsites -3 to +3. An in-depth analysis of each of these subsites gives an insight into the structure-function relationship of this enzyme and the basis of xylose/glucose discrimination in family 8 glycoside hydrolases. Furthermore, the structure of the -1/+1 subsite spanning complex reveals that the substrate is distorted from its ground state conformation. Indeed, structural analysis and in silico docking studies indicate that substrate hydrolysis in GH-8 members is preceded by a conformational change, away from the substrate ground-state chair conformation, to a pretransition state local minimum (2)S(O) conformation." @default.
• W1985440166 created "2016-06-24" @default.
• W1985440166 creator A5015838902 @default.
• W1985440166 creator A5016091464 @default.
• W1985440166 creator A5038526616 @default.
• W1985440166 creator A5042730297 @default.
• W1985440166 creator A5059310855 @default.
• W1985440166 creator A5066142303 @default.
• W1985440166 creator A5077001458 @default.
• W1985440166 creator A5077238999 @default.
• W1985440166 date "2006-03-25" @default.
• W1985440166 modified "2023-10-17" @default.
• W1985440166 title "Oligosaccharide Binding in Family 8 Glycosidases: Crystal Structures of Active-Site Mutants of the β-1,4-Xylanase pXyl from<i> Pseudoaltermonas haloplanktis</i> TAH3a in Complex with Substrate and Product^," @default.
• W1985440166 cites W1935712031 @default.
• W1985440166 cites W1975505188 @default.
• W1985440166 cites W1986191025 @default.
• W1985440166 cites W1988966516 @default.
• W1985440166 cites W2015080980 @default.
• W1985440166 cites W2023428170 @default.
• W1985440166 cites W2051628563 @default.
• W1985440166 cites W2065905475 @default.
• W1985440166 cites W2091075510 @default.
• W1985440166 cites W2104298650 @default.
• W1985440166 cites W2107025108 @default.
• W1985440166 cites W2108218166 @default.
• W1985440166 cites W2113964973 @default.
• W1985440166 cites W2115571077 @default.
• W1985440166 cites W2122199548 @default.
• W1985440166 cites W2123859926 @default.
• W1985440166 cites W2125077852 @default.
• W1985440166 cites W2143572860 @default.
• W1985440166 cites W2152756549 @default.
• W1985440166 cites W2158534713 @default.
• W1985440166 cites W2172165976 @default.
• W1985440166 cites W2950469006 @default.
• W1985440166 cites W607406955 @default.
• W1985440166 doi "https://doi.org/10.1021/bi052193e" @default.
• W1985440166 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16605248" @default.
• W1985440166 hasPublicationYear "2006" @default.
• W1985440166 type Work @default.
• W1985440166 sameAs 1985440166 @default.
• W1985440166 citedByCount "50" @default.
• W1985440166 countsByYear W19854401662012 @default.
• W1985440166 countsByYear W19854401662013 @default.
• W1985440166 countsByYear W19854401662014 @default.
• W1985440166 countsByYear W19854401662016 @default.
• W1985440166 countsByYear W19854401662017 @default.
• W1985440166 countsByYear W19854401662018 @default.
• W1985440166 countsByYear W19854401662019 @default.
• W1985440166 countsByYear W19854401662020 @default.
• W1985440166 countsByYear W19854401662021 @default.
• W1985440166 countsByYear W19854401662022 @default.
• W1985440166 countsByYear W19854401662023 @default.
• W1985440166 crossrefType "journal-article" @default.
• W1985440166 hasAuthorship W1985440166A5015838902 @default.
• W1985440166 hasAuthorship W1985440166A5016091464 @default.
• W1985440166 hasAuthorship W1985440166A5038526616 @default.
• W1985440166 hasAuthorship W1985440166A5042730297 @default.
• W1985440166 hasAuthorship W1985440166A5059310855 @default.
• W1985440166 hasAuthorship W1985440166A5066142303 @default.
• W1985440166 hasAuthorship W1985440166A5077001458 @default.
• W1985440166 hasAuthorship W1985440166A5077238999 @default.
• W1985440166 hasConcept C107824862 @default.
• W1985440166 hasConcept C116569031 @default.
• W1985440166 hasConcept C159110408 @default.
• W1985440166 hasConcept C170493617 @default.
• W1985440166 hasConcept C181199279 @default.
• W1985440166 hasConcept C185592680 @default.
• W1985440166 hasConcept C18903297 @default.
• W1985440166 hasConcept C196032511 @default.
• W1985440166 hasConcept C2777289219 @default.
• W1985440166 hasConcept C2777339483 @default.
• W1985440166 hasConcept C2780120296 @default.
• W1985440166 hasConcept C41183919 @default.
• W1985440166 hasConcept C41685203 @default.
• W1985440166 hasConcept C47701112 @default.
• W1985440166 hasConcept C55493867 @default.
• W1985440166 hasConcept C71240020 @default.
• W1985440166 hasConcept C71924100 @default.
• W1985440166 hasConcept C8010536 @default.
• W1985440166 hasConcept C86803240 @default.
• W1985440166 hasConceptScore W1985440166C107824862 @default.
• W1985440166 hasConceptScore W1985440166C116569031 @default.
• W1985440166 hasConceptScore W1985440166C159110408 @default.
• W1985440166 hasConceptScore W1985440166C170493617 @default.
• W1985440166 hasConceptScore W1985440166C181199279 @default.
• W1985440166 hasConceptScore W1985440166C185592680 @default.
• W1985440166 hasConceptScore W1985440166C18903297 @default.
• W1985440166 hasConceptScore W1985440166C196032511 @default.
• W1985440166 hasConceptScore W1985440166C2777289219 @default.
• W1985440166 hasConceptScore W1985440166C2777339483 @default.
• W1985440166 hasConceptScore W1985440166C2780120296 @default.
• W1985440166 hasConceptScore W1985440166C41183919 @default.
• W1985440166 hasConceptScore W1985440166C41685203 @default.
• W1985440166 hasConceptScore W1985440166C47701112 @default.
• W1985440166 hasConceptScore W1985440166C55493867 @default.
• W1985440166 hasConceptScore W1985440166C71240020 @default.
• W1985440166 hasConceptScore W1985440166C71924100 @default.

• next