FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985454080> ?p ?o ?g. }

• W1985454080 endingPage "3126" @default.
• W1985454080 startingPage "3121" @default.
• W1985454080 abstract "In this report, we demonstrate a label-free genosensor based on DNA hairpins coupled to gold coated sensor surfaces. The hairpin probes were labeled with a thiolated moiety for immobilization at the 5' end and with a fluorophore for signal transduction at the 3' end. In the absence of the complement, the fluorophore is quenched by energy transfer to the gold surface. Addition of the target sequence leads to the hairpin unfolding, and releases the fluorescent signal. This built-in property, using a gold film as both the immobilizing substrate and quenching agent, has the advantage of simplicity in design and ease of further integration. Our results showed that lengths of both the stem and the loop structures have significant effects on the sensor performance. Hybridization kinetics was investigated for various probe/target lengths and concentrations. An optimized hairpin probe gave a fluorescent signal increase of 39 folds after hybridization, which is much higher than the earlier reported results. A limit of detection (LOD) down to 0.3 nM for the complementary target DNA detection has been achieved. The developed sensor was further successfully applied for the detection of single-base mismatch targets, as well as for the direct detection of PCR products." @default.
• W1985454080 created "2016-06-24" @default.
• W1985454080 creator A5000173304 @default.
• W1985454080 creator A5006339665 @default.
• W1985454080 creator A5014128177 @default.
• W1985454080 creator A5022422612 @default.
• W1985454080 creator A5044057090 @default.
• W1985454080 creator A5053680126 @default.
• W1985454080 creator A5067635473 @default.
• W1985454080 creator A5069287014 @default.
• W1985454080 date "2011-03-01" @default.
• W1985454080 modified "2023-09-25" @default.
• W1985454080 title "Label-free genosensor based on immobilized DNA hairpins on gold surface" @default.
• W1985454080 cites W1981606423 @default.
• W1985454080 cites W1987970877 @default.
• W1985454080 cites W1993850231 @default.
• W1985454080 cites W1994338481 @default.
• W1985454080 cites W2013441540 @default.
• W1985454080 cites W2017359768 @default.
• W1985454080 cites W2018553797 @default.
• W1985454080 cites W2026710381 @default.
• W1985454080 cites W2033085688 @default.
• W1985454080 cites W2039231803 @default.
• W1985454080 cites W2044533903 @default.
• W1985454080 cites W2047418234 @default.
• W1985454080 cites W2049409956 @default.
• W1985454080 cites W2070168436 @default.
• W1985454080 cites W2071051523 @default.
• W1985454080 cites W2086941115 @default.
• W1985454080 cites W2093643675 @default.
• W1985454080 cites W2109007387 @default.
• W1985454080 cites W2116926903 @default.
• W1985454080 cites W2126012074 @default.
• W1985454080 cites W2133529983 @default.
• W1985454080 cites W2144511136 @default.
• W1985454080 cites W2160385392 @default.
• W1985454080 cites W2167452960 @default.
• W1985454080 doi "https://doi.org/10.1016/j.bios.2010.12.008" @default.
• W1985454080 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21208795" @default.
• W1985454080 hasPublicationYear "2011" @default.
• W1985454080 type Work @default.
• W1985454080 sameAs 1985454080 @default.
• W1985454080 citedByCount "14" @default.
• W1985454080 countsByYear W19854540802012 @default.
• W1985454080 countsByYear W19854540802013 @default.
• W1985454080 countsByYear W19854540802014 @default.
• W1985454080 countsByYear W19854540802015 @default.
• W1985454080 countsByYear W19854540802016 @default.
• W1985454080 countsByYear W19854540802017 @default.
• W1985454080 countsByYear W19854540802018 @default.
• W1985454080 countsByYear W19854540802020 @default.
• W1985454080 crossrefType "journal-article" @default.
• W1985454080 hasAuthorship W1985454080A5000173304 @default.
• W1985454080 hasAuthorship W1985454080A5006339665 @default.
• W1985454080 hasAuthorship W1985454080A5014128177 @default.
• W1985454080 hasAuthorship W1985454080A5022422612 @default.
• W1985454080 hasAuthorship W1985454080A5044057090 @default.
• W1985454080 hasAuthorship W1985454080A5053680126 @default.
• W1985454080 hasAuthorship W1985454080A5067635473 @default.
• W1985454080 hasAuthorship W1985454080A5069287014 @default.
• W1985454080 hasConcept C104819515 @default.
• W1985454080 hasConcept C119128265 @default.
• W1985454080 hasConcept C119203544 @default.
• W1985454080 hasConcept C120665830 @default.
• W1985454080 hasConcept C121332964 @default.
• W1985454080 hasConcept C12554922 @default.
• W1985454080 hasConcept C129312508 @default.
• W1985454080 hasConcept C155672457 @default.
• W1985454080 hasConcept C160756335 @default.
• W1985454080 hasConcept C171250308 @default.
• W1985454080 hasConcept C185592680 @default.
• W1985454080 hasConcept C18903297 @default.
• W1985454080 hasConcept C192562407 @default.
• W1985454080 hasConcept C21951064 @default.
• W1985454080 hasConcept C2776568683 @default.
• W1985454080 hasConcept C2777289219 @default.
• W1985454080 hasConcept C2778617687 @default.
• W1985454080 hasConcept C43617362 @default.
• W1985454080 hasConcept C552990157 @default.
• W1985454080 hasConcept C55493867 @default.
• W1985454080 hasConcept C71240020 @default.
• W1985454080 hasConcept C86803240 @default.
• W1985454080 hasConcept C91881484 @default.
• W1985454080 hasConceptScore W1985454080C104819515 @default.
• W1985454080 hasConceptScore W1985454080C119128265 @default.
• W1985454080 hasConceptScore W1985454080C119203544 @default.
• W1985454080 hasConceptScore W1985454080C120665830 @default.
• W1985454080 hasConceptScore W1985454080C121332964 @default.
• W1985454080 hasConceptScore W1985454080C12554922 @default.
• W1985454080 hasConceptScore W1985454080C129312508 @default.
• W1985454080 hasConceptScore W1985454080C155672457 @default.
• W1985454080 hasConceptScore W1985454080C160756335 @default.
• W1985454080 hasConceptScore W1985454080C171250308 @default.
• W1985454080 hasConceptScore W1985454080C185592680 @default.
• W1985454080 hasConceptScore W1985454080C18903297 @default.
• W1985454080 hasConceptScore W1985454080C192562407 @default.
• W1985454080 hasConceptScore W1985454080C21951064 @default.
• W1985454080 hasConceptScore W1985454080C2776568683 @default.

• next