FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985462714> ?p ?o ?g. }

• W1985462714 endingPage "e91220" @default.
• W1985462714 startingPage "e91220" @default.
• W1985462714 abstract "Upon irradiation with visible light, the photosensitizer-peptide conjugate eosin-(KLAKLAK)2 kills a broad spectrum of bacteria without damaging human cells. Eosin-(KLAKLAK)2 therefore represents an interesting lead compound for the treatment of local infection by photodynamic bacterial inactivation. The mechanisms of cellular killing by eosin-(KLAKLAK)2, however, remain unclear and this lack of knowledge hampers the development of optimized therapeutic agents. Herein, we investigate the localization of eosin-(KLAKLAK)2 in bacteria prior to light treatment and examine the molecular basis for the photodynamic activity of this conjugate.By employing photooxidation of 3,3-diaminobenzidine (DAB), (scanning) transmission electron microscopy ((S)TEM), and energy dispersive X-ray spectroscopy (EDS) methodologies, eosin-(KLAKLAK)2 is visualized at the surface of E. coli and S. aureus prior to photodynamic irradiation. Subsequent irradiation leads to severe membrane damage. Consistent with these observations, eosin-(KLAKLAK)2 binds to liposomes of bacterial lipid composition and causes liposomal leakage upon irradiation. The eosin moiety of the conjugate mediates bacterial killing and lipid bilayer leakage by generating the reactive oxygen species singlet oxygen and superoxide. In contrast, the (KLAKLAK)2 moiety targets the photosensitizer to bacterial lipid bilayers. In addition, while (KLAKLAK)2 does not disrupt intact liposomes, the peptide accelerates the leakage of photo-oxidized liposomes.Together, our results suggest that (KLAKLAK)2 promotes the binding of eosin Y to bacteria cell walls and lipid bilayers. Subsequent light irradiation results in membrane damage from the production of both Type I & II photodynamic products. Membrane damage by oxidation is then further aggravated by the (KLAKLAK)2 moiety and membrane lysis is accelerated by the peptide. These results therefore establish how photosensitizer and peptide act in synergy to achieve bacterial photo-inactivation. Learning how to exploit and optimize this synergy should lead to the development of future bacterial photoinactivation agents that are effective at low concentrations and at low light doses." @default.
• W1985462714 created "2016-06-24" @default.
• W1985462714 creator A5008429907 @default.
• W1985462714 creator A5011832960 @default.
• W1985462714 creator A5027719915 @default.
• W1985462714 creator A5031224601 @default.
• W1985462714 creator A5038823349 @default.
• W1985462714 creator A5088675982 @default.
• W1985462714 date "2014-03-07" @default.
• W1985462714 modified "2023-10-04" @default.
• W1985462714 title "Photoinduced Membrane Damage of E. coli and S. aureus by the Photosensitizer-Antimicrobial Peptide Conjugate Eosin-(KLAKLAK)2" @default.
• W1985462714 cites W1620588152 @default.
• W1985462714 cites W1622263065 @default.
• W1985462714 cites W1964893456 @default.
• W1985462714 cites W1965340064 @default.
• W1985462714 cites W1966417203 @default.
• W1985462714 cites W1967580039 @default.
• W1985462714 cites W1977192897 @default.
• W1985462714 cites W1978821331 @default.
• W1985462714 cites W1986529159 @default.
• W1985462714 cites W1986993803 @default.
• W1985462714 cites W1990733397 @default.
• W1985462714 cites W1995798370 @default.
• W1985462714 cites W1998411433 @default.
• W1985462714 cites W1998476066 @default.
• W1985462714 cites W2000857386 @default.
• W1985462714 cites W2008264122 @default.
• W1985462714 cites W2008461597 @default.
• W1985462714 cites W2012312802 @default.
• W1985462714 cites W2012490119 @default.
• W1985462714 cites W2013878893 @default.
• W1985462714 cites W2016343967 @default.
• W1985462714 cites W2017258688 @default.
• W1985462714 cites W2019098068 @default.
• W1985462714 cites W2023460725 @default.
• W1985462714 cites W2026805450 @default.
• W1985462714 cites W2026916202 @default.
• W1985462714 cites W2027839314 @default.
• W1985462714 cites W2027981241 @default.
• W1985462714 cites W2029682882 @default.
• W1985462714 cites W2031213053 @default.
• W1985462714 cites W2031720174 @default.
• W1985462714 cites W2031848920 @default.
• W1985462714 cites W2039128689 @default.
• W1985462714 cites W2041724207 @default.
• W1985462714 cites W2048737120 @default.
• W1985462714 cites W2054591216 @default.
• W1985462714 cites W2055776228 @default.
• W1985462714 cites W2060413674 @default.
• W1985462714 cites W2060758897 @default.
• W1985462714 cites W2066788247 @default.
• W1985462714 cites W2066937690 @default.
• W1985462714 cites W2069480047 @default.
• W1985462714 cites W2071581047 @default.
• W1985462714 cites W2072288954 @default.
• W1985462714 cites W2073737852 @default.
• W1985462714 cites W2074998353 @default.
• W1985462714 cites W2075892592 @default.
• W1985462714 cites W2084699782 @default.
• W1985462714 cites W2084845164 @default.
• W1985462714 cites W2086471725 @default.
• W1985462714 cites W2094389762 @default.
• W1985462714 cites W2095227440 @default.
• W1985462714 cites W2095407378 @default.
• W1985462714 cites W2098357857 @default.
• W1985462714 cites W2102404896 @default.
• W1985462714 cites W2107124262 @default.
• W1985462714 cites W2110528358 @default.
• W1985462714 cites W2112148102 @default.
• W1985462714 cites W2113483535 @default.
• W1985462714 cites W2115701223 @default.
• W1985462714 cites W2117690929 @default.
• W1985462714 cites W2132911648 @default.
• W1985462714 cites W2141222798 @default.
• W1985462714 cites W2144630525 @default.
• W1985462714 cites W2147173298 @default.
• W1985462714 cites W2147929482 @default.
• W1985462714 cites W2152747781 @default.
• W1985462714 cites W2154969258 @default.
• W1985462714 cites W2163059437 @default.
• W1985462714 cites W2163238015 @default.
• W1985462714 cites W2170265677 @default.
• W1985462714 cites W2187790984 @default.
• W1985462714 cites W2337428003 @default.
• W1985462714 cites W2491458126 @default.
• W1985462714 cites W2534696020 @default.
• W1985462714 cites W2790810025 @default.
• W1985462714 cites W51514869 @default.
• W1985462714 cites W91579775 @default.
• W1985462714 doi "https://doi.org/10.1371/journal.pone.0091220" @default.
• W1985462714 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3946741" @default.
• W1985462714 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24608860" @default.
• W1985462714 hasPublicationYear "2014" @default.
• W1985462714 type Work @default.
• W1985462714 sameAs 1985462714 @default.
• W1985462714 citedByCount "35" @default.
• W1985462714 countsByYear W19854627142015 @default.
• W1985462714 countsByYear W19854627142016 @default.

• next