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- W1985470010 abstract "The 1.2-kb DNA sequence flanking the transcription start of the AdoHcy hydrolase gene was cloned into the luciferase reporter plasmid pGL3-basic, and promoter activity was measured in transiently transfected CHO cells. Deletion analysis showed that most promoter activity was located within a 153 bp fragment immediately upstream from the predominant transcription start. The 153 bp fragment includes sites for AP-2, glucocorticoid-responsive element, SP-1, and a TATA-like sequence TATTTAAA. Mutational analysis demonstrated that the SP-1 site nearest the start of transcription contributed significantly to promoter activity, whereas, the other elements, including the appropriately positioned TATTTAAA sequence, had little affect on promoter activity." @default.
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- W1985470010 date "1997-11-01" @default.
- W1985470010 modified "2023-10-18" @default.
- W1985470010 title "The RatS-Adenosylhomocysteine Hydrolase Promoter" @default.
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- W1985470010 doi "https://doi.org/10.1006/bbrc.1997.7704" @default.
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