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- W1985498546 abstract "Cells require ribonucleotide reductase (RNR) activity for DNA replication. In bacteria, electrons can flow from NADPH to RNR by either a thioredoxin-reductase- or a glutathione-reductase-dependent route. Yeast and plants artificially lacking thioredoxin reductases exhibit a slow-growth phenotype, suggesting glutathione-reductase-dependent routes are poor at supporting DNA replication in these organisms. We have studied proliferation of thioredoxin-reductase-1 (Txnrd1)-deficient hepatocytes in mice. During development and regeneration, normal mice and mice having Txnrd1-deficient hepatocytes exhibited similar liver growth rates. Proportions of hepatocytes that immunostained for PCNA, phosphohistone H3 or incorporated BrdU were also similar, indicating livers of either genotype had similar levels of proliferative, S and M phase hepatocytes, respectively. Replication was blocked by hydroxyurea, confirming that RNR activity was required by Txnrd1-deficient hepatocytes. Regenerative thymidine incorporation was similar in normal and Txnrd1-deficient livers, further indicating that DNA synthesis was unaffected. Using genetic chimeras in which a fluorescently marked subset of hepatocytes was Txnrd1-deficient while others were not, we found that the multigenerational contributions of both hepatocyte types to development and to liver regeneration were indistinguishable. We conclude that, in mouse hepatocytes, a Txnrd1-independent route for the supply of electrons to RNR can fully support DNA replication and normal proliferative growth." @default.
- W1985498546 created "2016-06-24" @default.
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- W1985498546 date "2010-07-15" @default.
- W1985498546 modified "2023-10-14" @default.
- W1985498546 title "Hepatocytes lacking thioredoxin reductase 1 have normal replicative potential during development and regeneration" @default.
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- W1985498546 doi "https://doi.org/10.1242/jcs.068106" @default.
- W1985498546 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2894656" @default.
- W1985498546 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20571049" @default.
- W1985498546 hasPublicationYear "2010" @default.
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