FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985585221> ?p ?o ?g. }

• W1985585221 endingPage "5860" @default.
• W1985585221 startingPage "5851" @default.
• W1985585221 abstract "Human brain proteins containing phosphorus, copper, and zinc were detected directly in protein spots in gels of a human brain sample after separation by two-dimensional gel electrophoresis using laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS). A powerful laser ablation system with cooled laser ablation chamber was coupled to a double-focusing sector field ICPMS. The separated protein spots in 2D gels were fast screened using the optimized microanalytical LA-ICPMS technique measured at medium mass resolution with a focused laser beam (wavelength, 213 nm; diameter of laser crater, 50 μm; and laser power density, 3 × 109 W cm-2) with respect to selected three essential elements. Of 176 protein spots in 2D gel from a human brain sample, phosphorus, copper, and zinc were detected in 31, 43, and 49 protein spots, respectively. For the first time, uranium as a naturally occurring radioactive element was found in 20 selected protein spots. The detection limits for P, S, Cu, Zn and U were determined in singular protein spots with 0.0013, 1.29, 0.029, 0.063, and 0.000 01 mg g-1, respectively. A combination of LA-ICPMS with matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR-MS) was applied for the identification of selected protein spots from human brain protein separated by 2D gel electrophoresis. Combining MALDI-FTICR-MS for the structure analysis of metal- and phosphorus-containing human brain proteins with LA-ICPMS, the direct analysis of heteroelements on separated proteins in 2D gels can be performed. For quantification of analytical LA-ICPMS data, the number of sulfur atoms per protein (and following the sulfur concentration) determined by MALDI-FTICR-MS was used for internal standardization. From the known sulfur concentration in protein, the concentration of other heteroelements was calculated. In addition, the number of phosphorylation and the phosphorylation sites of phosphorylated proteins in the human brain sample detected by LA-ICPMS were determined by MALDI-FTICR-MS. This technique allows the study of posttranslational modifications in human brain proteins." @default.
• W1985585221 created "2016-06-24" @default.
• W1985585221 creator A5004832020 @default.
• W1985585221 creator A5011989259 @default.
• W1985585221 creator A5029317883 @default.
• W1985585221 creator A5059102429 @default.
• W1985585221 creator A5063939096 @default.
• W1985585221 creator A5074358365 @default.
• W1985585221 creator A5084703314 @default.
• W1985585221 creator A5090868751 @default.
• W1985585221 date "2005-08-19" @default.
• W1985585221 modified "2023-10-03" @default.
• W1985585221 title "Determination of Phosphorus-, Copper-, and Zinc-Containing Human Brain Proteins by LA-ICPMS and MALDI-FTICR-MS" @default.
• W1985585221 cites W120204100 @default.
• W1985585221 cites W1487645896 @default.
• W1985585221 cites W1969269523 @default.
• W1985585221 cites W1974549278 @default.
• W1985585221 cites W1982188730 @default.
• W1985585221 cites W1989560433 @default.
• W1985585221 cites W1990543458 @default.
• W1985585221 cites W1993305231 @default.
• W1985585221 cites W1994710255 @default.
• W1985585221 cites W2004014728 @default.
• W1985585221 cites W2008028760 @default.
• W1985585221 cites W2009362333 @default.
• W1985585221 cites W2021359521 @default.
• W1985585221 cites W2039749194 @default.
• W1985585221 cites W2046316900 @default.
• W1985585221 cites W2050648941 @default.
• W1985585221 cites W2051516838 @default.
• W1985585221 cites W2057210351 @default.
• W1985585221 cites W2058170418 @default.
• W1985585221 cites W2058548939 @default.
• W1985585221 cites W2070228646 @default.
• W1985585221 cites W2077697165 @default.
• W1985585221 cites W2100837269 @default.
• W1985585221 doi "https://doi.org/10.1021/ac0506579" @default.
• W1985585221 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16159114" @default.
• W1985585221 hasPublicationYear "2005" @default.
• W1985585221 type Work @default.
• W1985585221 sameAs 1985585221 @default.
• W1985585221 citedByCount "78" @default.
• W1985585221 countsByYear W19855852212012 @default.
• W1985585221 countsByYear W19855852212013 @default.
• W1985585221 countsByYear W19855852212014 @default.
• W1985585221 countsByYear W19855852212015 @default.
• W1985585221 countsByYear W19855852212016 @default.
• W1985585221 countsByYear W19855852212017 @default.
• W1985585221 countsByYear W19855852212018 @default.
• W1985585221 countsByYear W19855852212019 @default.
• W1985585221 countsByYear W19855852212020 @default.
• W1985585221 crossrefType "journal-article" @default.
• W1985585221 hasAuthorship W1985585221A5004832020 @default.
• W1985585221 hasAuthorship W1985585221A5011989259 @default.
• W1985585221 hasAuthorship W1985585221A5029317883 @default.
• W1985585221 hasAuthorship W1985585221A5059102429 @default.
• W1985585221 hasAuthorship W1985585221A5063939096 @default.
• W1985585221 hasAuthorship W1985585221A5074358365 @default.
• W1985585221 hasAuthorship W1985585221A5084703314 @default.
• W1985585221 hasAuthorship W1985585221A5090868751 @default.
• W1985585221 hasBestOaLocation W19855852212 @default.
• W1985585221 hasConcept C113196181 @default.
• W1985585221 hasConcept C120665830 @default.
• W1985585221 hasConcept C121332964 @default.
• W1985585221 hasConcept C150394285 @default.
• W1985585221 hasConcept C162356407 @default.
• W1985585221 hasConcept C162711632 @default.
• W1985585221 hasConcept C170552419 @default.
• W1985585221 hasConcept C178790620 @default.
• W1985585221 hasConcept C185592680 @default.
• W1985585221 hasConcept C2779188808 @default.
• W1985585221 hasConcept C43617362 @default.
• W1985585221 hasConcept C520434653 @default.
• W1985585221 hasConcept C535196362 @default.
• W1985585221 hasConcept C75280812 @default.
• W1985585221 hasConceptScore W1985585221C113196181 @default.
• W1985585221 hasConceptScore W1985585221C120665830 @default.
• W1985585221 hasConceptScore W1985585221C121332964 @default.
• W1985585221 hasConceptScore W1985585221C150394285 @default.
• W1985585221 hasConceptScore W1985585221C162356407 @default.
• W1985585221 hasConceptScore W1985585221C162711632 @default.
• W1985585221 hasConceptScore W1985585221C170552419 @default.
• W1985585221 hasConceptScore W1985585221C178790620 @default.
• W1985585221 hasConceptScore W1985585221C185592680 @default.
• W1985585221 hasConceptScore W1985585221C2779188808 @default.
• W1985585221 hasConceptScore W1985585221C43617362 @default.
• W1985585221 hasConceptScore W1985585221C520434653 @default.
• W1985585221 hasConceptScore W1985585221C535196362 @default.
• W1985585221 hasConceptScore W1985585221C75280812 @default.
• W1985585221 hasIssue "18" @default.
• W1985585221 hasLocation W19855852211 @default.
• W1985585221 hasLocation W19855852212 @default.
• W1985585221 hasLocation W19855852213 @default.
• W1985585221 hasLocation W19855852214 @default.
• W1985585221 hasOpenAccess W1985585221 @default.
• W1985585221 hasPrimaryLocation W19855852211 @default.
• W1985585221 hasRelatedWork W1989237022 @default.
• W1985585221 hasRelatedWork W2069636393 @default.

• next