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- W1985588986 abstract "DNA extracted from the Escherichia coli bacteriophage P2, as studied in the electron microscope, consists of linear monomers of uniform length (13.1 to 13.2 μ, corresponding to a molecular weight of 2.2 × 107), in addition to dimers and circles. These disappear when the temperature is raised just above that at which denaturation begins. The presence in P2 DNA of cohesive ends like those described for phage λ DNA is thus confirmed. A direct comparison with λ DNA in the electron microscope, and the high temperature at which P2 DNA infectivity is recovered, both show that P2 DNA cohesive ends are much more stable to heat than those of λ. Electron microscopy of partially denatured P2 DNA reveals several highly characteristic zones along the DNA “melting map”, recognizable by the temperature at which they start denaturing. Changes in ultraviolet absorption of P2 DNA at 260 nm wavelength during heat denaturation (melting curves), and also the accompanying spectral changes, indicate that P2 DNA contains at least three fractions of different base pair contents (fraction I: 20% of mass with 34% (G + C) content; II: 16% with 45% (G + C); III: 64% with 58% (G + C). The electron microscopical observations permit one to conclude that this compositional heterogeneity is intra-molecular. A correlation between the DNA fractions defined by the melting curves and the denaturation zones of the melting map is tentatively established. The DNA of P2 Hy, a phage believed to be genetically identical to P2 except for a small segment of its chromosome, appears to be identical to P2 except that fraction I is over-represented in P2 Hy DNA. The electron microscopic data are compatible with this finding, but the effect is very small. On this basis it is possible tentatively to localize on the melting map of P2 DNA the genetic region which differentiates it from P2 Hy, and thereby orient the physical in respect to the genetic representation of the P2 chromosome." @default.
- W1985588986 created "2016-06-24" @default.
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- W1985588986 date "1969-09-01" @default.
- W1985588986 modified "2023-10-16" @default.
- W1985588986 title "Heat denaturation of P2 bacteriophage DNA: Compositional heterogeneity" @default.
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- W1985588986 doi "https://doi.org/10.1016/0022-2836(69)90378-7" @default.
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