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- W1985647599 abstract "ABSTRACT Reovirus replication occurs in the cytoplasm of infected cells and culminates in the formation of crystalline arrays of progeny virions within viral inclusions. Two viral nonstructural proteins, σNS and μNS, and structural protein σ3 form protein-RNA complexes early in reovirus infection. To better understand the minimal requirements of viral inclusion formation, we expressed σNS, μNS, and σ3 alone and in combination in the absence of viral infection. In contrast to its concentration in inclusion structures during reovirus replication, σNS expressed in cells in the absence of infection is distributed diffusely throughout the cytoplasm and does not form structures that resemble viral inclusions. Expressed σNS is functional as it complements the defect in temperature-sensitive, σNS-mutant virus ts E320. In both transfected and infected cells, μNS is found in punctate cytoplasmic structures and σ3 is distributed diffusely in the cytoplasm and the nucleus. The subcellular localization of μNS and σ3 is not altered when the proteins are expressed together or with σNS. However, when expressed with μNS, σNS colocalizes with μNS to punctate structures similar in morphology to inclusion structures observed early in viral replication. During reovirus infection, both σNS and μNS are detectable 4 h after adsorption and colocalize to punctate structures throughout the viral life cycle. In concordance with these results, σNS interacts with μNS in a yeast two-hybrid assay and by coimmunoprecipitation analysis. These data suggest that σNS and μNS are the minimal viral components required to form inclusions, which then recruit other reovirus proteins and RNA to initiate viral genome replication." @default.
- W1985647599 created "2016-06-24" @default.
- W1985647599 creator A5003172554 @default.
- W1985647599 creator A5021717248 @default.
- W1985647599 creator A5023812277 @default.
- W1985647599 date "2003-05-15" @default.
- W1985647599 modified "2023-09-27" @default.
- W1985647599 title "Reovirus σNS and μNS Proteins Form Cytoplasmic Inclusion Structures in the Absence of Viral Infection" @default.
- W1985647599 cites W1553034097 @default.
- W1985647599 cites W1554199218 @default.
- W1985647599 cites W1554569594 @default.
- W1985647599 cites W1560660711 @default.
- W1985647599 cites W1568572766 @default.
- W1985647599 cites W1574028564 @default.
- W1985647599 cites W1578184340 @default.
- W1985647599 cites W1586927519 @default.
- W1985647599 cites W1599490590 @default.
- W1985647599 cites W1600788583 @default.
- W1985647599 cites W1652595607 @default.
- W1985647599 cites W1654444165 @default.
- W1985647599 cites W1755581022 @default.
- W1985647599 cites W1779477936 @default.
- W1985647599 cites W1824110272 @default.
- W1985647599 cites W1836613779 @default.
- W1985647599 cites W1843991682 @default.
- W1985647599 cites W1867247140 @default.
- W1985647599 cites W1873940247 @default.
- W1985647599 cites W1899984563 @default.
- W1985647599 cites W1964317873 @default.
- W1985647599 cites W1966455695 @default.
- W1985647599 cites W1969261348 @default.
- W1985647599 cites W1972160946 @default.
- W1985647599 cites W1973088913 @default.
- W1985647599 cites W1973759974 @default.
- W1985647599 cites W1975730655 @default.
- W1985647599 cites W1979969348 @default.
- W1985647599 cites W1980806210 @default.
- W1985647599 cites W1980929865 @default.
- W1985647599 cites W1982691720 @default.
- W1985647599 cites W1983407779 @default.
- W1985647599 cites W1984534463 @default.
- W1985647599 cites W1984873948 @default.
- W1985647599 cites W1985369047 @default.
- W1985647599 cites W1990640355 @default.
- W1985647599 cites W1996550262 @default.
- W1985647599 cites W1997944487 @default.
- W1985647599 cites W2000085886 @default.
- W1985647599 cites W2001213608 @default.
- W1985647599 cites W2001659773 @default.
- W1985647599 cites W2002696097 @default.
- W1985647599 cites W2004348784 @default.
- W1985647599 cites W2006331766 @default.
- W1985647599 cites W2008623396 @default.
- W1985647599 cites W2008781481 @default.
- W1985647599 cites W2008892454 @default.
- W1985647599 cites W2012657116 @default.
- W1985647599 cites W2019475043 @default.
- W1985647599 cites W2021189921 @default.
- W1985647599 cites W2022712238 @default.
- W1985647599 cites W2023165989 @default.
- W1985647599 cites W2023251669 @default.
- W1985647599 cites W2023270632 @default.
- W1985647599 cites W2023655582 @default.
- W1985647599 cites W2026498986 @default.
- W1985647599 cites W2028124746 @default.
- W1985647599 cites W2028658614 @default.
- W1985647599 cites W2030785017 @default.
- W1985647599 cites W2030849392 @default.
- W1985647599 cites W2031757923 @default.
- W1985647599 cites W2032148650 @default.
- W1985647599 cites W2045839299 @default.
- W1985647599 cites W2050475218 @default.
- W1985647599 cites W2052136939 @default.
- W1985647599 cites W2052603372 @default.
- W1985647599 cites W2053000264 @default.
- W1985647599 cites W2056836205 @default.
- W1985647599 cites W2057302079 @default.
- W1985647599 cites W2060385149 @default.
- W1985647599 cites W2061242081 @default.
- W1985647599 cites W2064943509 @default.
- W1985647599 cites W2066705273 @default.
- W1985647599 cites W2067597530 @default.
- W1985647599 cites W2072963880 @default.
- W1985647599 cites W2074356416 @default.
- W1985647599 cites W2077588626 @default.
- W1985647599 cites W2078902140 @default.
- W1985647599 cites W2079877919 @default.
- W1985647599 cites W2085864918 @default.
- W1985647599 cites W2090270095 @default.
- W1985647599 cites W2092199562 @default.
- W1985647599 cites W2105985022 @default.
- W1985647599 cites W2110811665 @default.
- W1985647599 cites W2115094328 @default.
- W1985647599 cites W2116380451 @default.
- W1985647599 cites W2119809007 @default.
- W1985647599 cites W2120350522 @default.
- W1985647599 cites W2122776465 @default.
- W1985647599 cites W2126639542 @default.