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• W1985694539 abstract "TALEN-based inactivation of the zebrafish pak4 gene resulted in embryos and adult fish that appear normal and fertile. This is in contrast to our previously published studies which were based on the use of antisense morpholino oligonucleotides (MOs). We have excluded potential explanations such as gene duplication, alternate splicing, cryptic initiation of translation, and translation-independent RNA function. Our conclusion is that pak4 is dispensable in zebrafish, and that even when corroborated by robust controls, such as RNA rescue, MOs may elicit misleading pseudophenotypes that do not correspond to results obtained by genetic mutations, and should thus be used with caution." @default.
• W1985694539 created "2016-06-24" @default.
• W1985694539 creator A5018516469 @default.
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• W1985694539 date "2014-06-19" @default.
• W1985694539 modified "2023-10-16" @default.
• W1985694539 title "The Serine-Threonine Protein Kinase PAK4 Is Dispensable in Zebrafish: Identification of a Morpholino-Generated Pseudophenotype" @default.
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• W1985694539 doi "https://doi.org/10.1371/journal.pone.0100268" @default.
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