Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985697913> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W1985697913 endingPage "1037" @default.
- W1985697913 startingPage "1026" @default.
- W1985697913 abstract "Streptomyces kasugaensis G3 was transformed by pIJ702 DNA carrying the thiostrepton-resistance gene at a frequency of 2×105 transformants/μg DNA, but it was found that the introduced pIJ702 was very unstable in this strain. This result led us to make useful vectors using the stable plasmids resident in S. kasugaensis. The Bcl I-fragment, containing the thiostrepton-resistance gene obtained from pIJ702, was inserted into the pSK1 and pSK2 plasmids isolated from S. kasugaensis. Two composite plasmids, pSK11-1 (8.0 Md) and pSK21-1 (4.8 Md), were isolated from the thiostrepton-resistant transformants of strain G3. The constructed pSK11-1 consisted of the entire pSK1 molecule and the thiostrepton-resistance gene fragment. pSK21-1 consisted of the large Bcl I-fragment of pSK2 (4.1 Md) and the same thiostrepton-resistance gene. These plasmids were stably maintained in S. kasugaensis G3. Small derivatives of these composite plasmids were prepared by restriction enzyme cleavage and self-ligation, and several unique insertion sites were also constructed in these small plasmids. By analysis of the physical maps of these plasmids, the essential regions of pSK1 and pSK2 were determined from their DNA segments to be 2.5 Md in Psk11-1 and 1.9 Md in pSK21-1. pSK21-B5, one of these plasmid vectors, showed a wide host range in the genus Streptomyces and was stable maintained in all streptomycete species tested, except S. kasugaensis M338." @default.
- W1985697913 created "2016-06-24" @default.
- W1985697913 creator A5011170532 @default.
- W1985697913 creator A5016129359 @default.
- W1985697913 creator A5081838477 @default.
- W1985697913 date "1984-01-01" @default.
- W1985697913 modified "2023-10-02" @default.
- W1985697913 title "Construction of plasmid vectors from Streptomyces kasugaensis plasmids, pSK1 and pSK2." @default.
- W1985697913 cites W1595419726 @default.
- W1985697913 cites W1651491538 @default.
- W1985697913 cites W1967833770 @default.
- W1985697913 cites W1974024998 @default.
- W1985697913 cites W1982760829 @default.
- W1985697913 cites W1998870034 @default.
- W1985697913 cites W2002008305 @default.
- W1985697913 cites W2006283042 @default.
- W1985697913 cites W2012924024 @default.
- W1985697913 cites W2027118671 @default.
- W1985697913 cites W2029432415 @default.
- W1985697913 cites W2047742221 @default.
- W1985697913 cites W2064815233 @default.
- W1985697913 cites W2074820260 @default.
- W1985697913 cites W2140778384 @default.
- W1985697913 cites W2152431828 @default.
- W1985697913 cites W2412729950 @default.
- W1985697913 doi "https://doi.org/10.7164/antibiotics.37.1026" @default.
- W1985697913 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6094413" @default.
- W1985697913 hasPublicationYear "1984" @default.
- W1985697913 type Work @default.
- W1985697913 sameAs 1985697913 @default.
- W1985697913 citedByCount "16" @default.
- W1985697913 countsByYear W19856979132012 @default.
- W1985697913 countsByYear W19856979132014 @default.
- W1985697913 crossrefType "journal-article" @default.
- W1985697913 hasAuthorship W1985697913A5011170532 @default.
- W1985697913 hasAuthorship W1985697913A5016129359 @default.
- W1985697913 hasAuthorship W1985697913A5081838477 @default.
- W1985697913 hasBestOaLocation W19856979131 @default.
- W1985697913 hasConcept C104317684 @default.
- W1985697913 hasConcept C109932267 @default.
- W1985697913 hasConcept C128319531 @default.
- W1985697913 hasConcept C153911025 @default.
- W1985697913 hasConcept C205225487 @default.
- W1985697913 hasConcept C22744801 @default.
- W1985697913 hasConcept C2777578258 @default.
- W1985697913 hasConcept C2779920670 @default.
- W1985697913 hasConcept C523546767 @default.
- W1985697913 hasConcept C54355233 @default.
- W1985697913 hasConcept C552990157 @default.
- W1985697913 hasConcept C67705224 @default.
- W1985697913 hasConcept C86803240 @default.
- W1985697913 hasConcept C88478588 @default.
- W1985697913 hasConcept C89423630 @default.
- W1985697913 hasConceptScore W1985697913C104317684 @default.
- W1985697913 hasConceptScore W1985697913C109932267 @default.
- W1985697913 hasConceptScore W1985697913C128319531 @default.
- W1985697913 hasConceptScore W1985697913C153911025 @default.
- W1985697913 hasConceptScore W1985697913C205225487 @default.
- W1985697913 hasConceptScore W1985697913C22744801 @default.
- W1985697913 hasConceptScore W1985697913C2777578258 @default.
- W1985697913 hasConceptScore W1985697913C2779920670 @default.
- W1985697913 hasConceptScore W1985697913C523546767 @default.
- W1985697913 hasConceptScore W1985697913C54355233 @default.
- W1985697913 hasConceptScore W1985697913C552990157 @default.
- W1985697913 hasConceptScore W1985697913C67705224 @default.
- W1985697913 hasConceptScore W1985697913C86803240 @default.
- W1985697913 hasConceptScore W1985697913C88478588 @default.
- W1985697913 hasConceptScore W1985697913C89423630 @default.
- W1985697913 hasIssue "9" @default.
- W1985697913 hasLocation W19856979131 @default.
- W1985697913 hasLocation W19856979132 @default.
- W1985697913 hasOpenAccess W1985697913 @default.
- W1985697913 hasPrimaryLocation W19856979131 @default.
- W1985697913 hasRelatedWork W1968312083 @default.
- W1985697913 hasRelatedWork W1975675671 @default.
- W1985697913 hasRelatedWork W1985697913 @default.
- W1985697913 hasRelatedWork W2007982609 @default.
- W1985697913 hasRelatedWork W2019410656 @default.
- W1985697913 hasRelatedWork W2061649476 @default.
- W1985697913 hasRelatedWork W2082546987 @default.
- W1985697913 hasRelatedWork W2144609449 @default.
- W1985697913 hasRelatedWork W2466192070 @default.
- W1985697913 hasRelatedWork W3023663517 @default.
- W1985697913 hasVolume "37" @default.
- W1985697913 isParatext "false" @default.
- W1985697913 isRetracted "false" @default.
- W1985697913 magId "1985697913" @default.
- W1985697913 workType "article" @default.