FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985706963> ?p ?o ?g. }

• W1985706963 endingPage "395" @default.
• W1985706963 startingPage "386" @default.
• W1985706963 abstract "At present, the assessment of the allergenic potential of chemicals is carried out using animal models. Over the last decade, several in vitro methods mainly using primary dendritic cells have been proposed to identify the potential of chemicals to induce skin sensitization to meet current animal welfare and public opinions. The major limitations of such tests are the donor-to-donor variability, the low levels in the source, and a possible shortage of human sources. The aim of the present investigation was to establish an in vitro test to identify chemical allergens using the human promyelocytic cell line THP-1 in order to avoid some of these difficulties. We investigated whether the chemokine interleukin-8 or CXCL8 (IL-8) production could provide a methodology for the detection of both respiratory and contact allergens. THP-1 cells were exposed to contact allergens (cinnamaldehyde, dinitrochlorobenzene, nickel sulfate, penicillin G, p-phenylenediamine, tetramethylthiuram disulfide), to respiratory allergens (ammonium hexachloroplatinate, diphenylmethane diisocyanate, trimellitic anhydride) and to irritants (salicylic acid, phenol, sodium lauryl sulphate). Following 48 h of incubation, the release of IL-8 was evaluated by sandwich ELISA. IL-8 production was significantly increased after stimulation with all allergens tested, with the exception of trimellitic anhydride, whereas irritants exposure failed to induce IL-8 release. The lack of IL-8 production by trimellitic anhydride can be explained by the rapid hydrolysis of this chemical in water to trimellitic acid, which is not an allergen. In contrast to IL-8 release, CD54 and CD86 expression did not provide a sensitive method failing to correctly identify approximately 30% of the tested compounds. Although CD86 appears to be a more sensitive marker than CD54 when discriminating allergens from irritants neither of these markers provided robust methodology. We also investigated if a common activation pathway in allergen-induced IL-8 production involving p38 mitogen-activated protein kinase could be identified. By Western blot analysis we could indeed demonstrate p38 activation by all chemical allergens tested and, using the selective p38 MAPK inhibitor SB203580, a significant modulation of allergen-induced IL-8 release could be achieved in all cases. Our data suggests that production of IL-8 by naïve THP-1 cells may represent a promising in vitro model for the screening of potential chemical allergens and activation of p38 MAPK represents a common pathway triggered by allergens." @default.
• W1985706963 created "2016-06-24" @default.
• W1985706963 creator A5018197046 @default.
• W1985706963 creator A5024026169 @default.
• W1985706963 creator A5032455971 @default.
• W1985706963 creator A5051874658 @default.
• W1985706963 creator A5063639436 @default.
• W1985706963 creator A5082724673 @default.
• W1985706963 date "2008-03-01" @default.
• W1985706963 modified "2023-10-18" @default.
• W1985706963 title "Role of p38 MAPK in the selective release of IL-8 induced by chemical allergen in naïve THP-1 cells" @default.
• W1985706963 cites W1499902555 @default.
• W1985706963 cites W1553847148 @default.
• W1985706963 cites W1753071495 @default.
• W1985706963 cites W1753762092 @default.
• W1985706963 cites W1964149642 @default.
• W1985706963 cites W1965161755 @default.
• W1985706963 cites W1969463935 @default.
• W1985706963 cites W1975228050 @default.
• W1985706963 cites W1978245770 @default.
• W1985706963 cites W1999209207 @default.
• W1985706963 cites W2002679351 @default.
• W1985706963 cites W2015696927 @default.
• W1985706963 cites W2017092296 @default.
• W1985706963 cites W2026140714 @default.
• W1985706963 cites W2028598695 @default.
• W1985706963 cites W2028808592 @default.
• W1985706963 cites W2048389635 @default.
• W1985706963 cites W2053247945 @default.
• W1985706963 cites W2057776973 @default.
• W1985706963 cites W2065012084 @default.
• W1985706963 cites W2081733990 @default.
• W1985706963 cites W2085596394 @default.
• W1985706963 cites W2086790843 @default.
• W1985706963 cites W2093682600 @default.
• W1985706963 cites W2111220263 @default.
• W1985706963 cites W2141505644 @default.
• W1985706963 cites W2156936329 @default.
• W1985706963 cites W2236421686 @default.
• W1985706963 cites W2408724080 @default.
• W1985706963 doi "https://doi.org/10.1016/j.tiv.2007.10.005" @default.
• W1985706963 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18494145" @default.
• W1985706963 hasPublicationYear "2008" @default.
• W1985706963 type Work @default.
• W1985706963 sameAs 1985706963 @default.
• W1985706963 citedByCount "66" @default.
• W1985706963 countsByYear W19857069632012 @default.
• W1985706963 countsByYear W19857069632013 @default.
• W1985706963 countsByYear W19857069632014 @default.
• W1985706963 countsByYear W19857069632015 @default.
• W1985706963 countsByYear W19857069632016 @default.
• W1985706963 countsByYear W19857069632017 @default.
• W1985706963 countsByYear W19857069632018 @default.
• W1985706963 countsByYear W19857069632019 @default.
• W1985706963 countsByYear W19857069632020 @default.
• W1985706963 countsByYear W19857069632022 @default.
• W1985706963 countsByYear W19857069632023 @default.
• W1985706963 crossrefType "journal-article" @default.
• W1985706963 hasAuthorship W1985706963A5018197046 @default.
• W1985706963 hasAuthorship W1985706963A5024026169 @default.
• W1985706963 hasAuthorship W1985706963A5032455971 @default.
• W1985706963 hasAuthorship W1985706963A5051874658 @default.
• W1985706963 hasAuthorship W1985706963A5063639436 @default.
• W1985706963 hasAuthorship W1985706963A5082724673 @default.
• W1985706963 hasConcept C178790620 @default.
• W1985706963 hasConcept C185592680 @default.
• W1985706963 hasConcept C2780373086 @default.
• W1985706963 hasConcept C2781167081 @default.
• W1985706963 hasConcept C29730261 @default.
• W1985706963 hasConcept C55493867 @default.
• W1985706963 hasConceptScore W1985706963C178790620 @default.
• W1985706963 hasConceptScore W1985706963C185592680 @default.
• W1985706963 hasConceptScore W1985706963C2780373086 @default.
• W1985706963 hasConceptScore W1985706963C2781167081 @default.
• W1985706963 hasConceptScore W1985706963C29730261 @default.
• W1985706963 hasConceptScore W1985706963C55493867 @default.
• W1985706963 hasIssue "2" @default.
• W1985706963 hasLocation W19857069631 @default.
• W1985706963 hasLocation W19857069632 @default.
• W1985706963 hasOpenAccess W1985706963 @default.
• W1985706963 hasPrimaryLocation W19857069631 @default.
• W1985706963 hasRelatedWork W1531601525 @default.
• W1985706963 hasRelatedWork W1985706963 @default.
• W1985706963 hasRelatedWork W2036824743 @default.
• W1985706963 hasRelatedWork W2062294563 @default.
• W1985706963 hasRelatedWork W2396946412 @default.
• W1985706963 hasRelatedWork W2748952813 @default.
• W1985706963 hasRelatedWork W2899084033 @default.
• W1985706963 hasRelatedWork W2948807893 @default.
• W1985706963 hasRelatedWork W4387497383 @default.
• W1985706963 hasRelatedWork W2778153218 @default.
• W1985706963 hasVolume "22" @default.
• W1985706963 isParatext "false" @default.
• W1985706963 isRetracted "false" @default.
• W1985706963 magId "1985706963" @default.
• W1985706963 workType "article" @default.