Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985728844> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W1985728844 endingPage "1972" @default.
- W1985728844 startingPage "1967" @default.
- W1985728844 abstract "In the present investigation, a method of studying the maximal rate of superoxide anion (O2·−) production in immobilised human neutrophils using a microtiter plate technique has been developed. The rate of O2·− production was determined from the rate of reduction of cytochrome (III) C, studied as the increase in absorbance at 550 nm. The protein kinase C activator, phorbol 12-myristate 13-acetate, was used to stimulate O2·− production. Neutrophils were evenly immobilised as a monolayer to microtiter culture plates to provide a reproducible exposure to the medium. Phorbol ester stimulated O2·− production was inhibited by staurosporine, a well-known inhibitor of protein kinase C, and by diphenylene iodonium, a potent NADPH-oxidase inhibitor, with ic50-values in this assay of 20 and 220 nm, respectively. The extracellularly produced O2·− was removed by superoxide dismutase with a half maximal effect of 0.6 μg/mL. The maximal production rate of O2·− could therefore be estimated by addition of 20 μg/mL superoxide dismutase. Several antioxidants, including butylated hydroxytoluene, nordihydroguairetic acid, probucol and α-tocopherol, were studied and showed neither an effect on O2·− production nor a scavenging effect. This new method was highly reproducible, and the continuous measurement of O2·− production was very useful for validating the effect of inhibitors. The developed microtiter technique using immobilised cells has a large capacity and allows different compounds to be tested under comparable conditions, since they are exposed to the cells in a similar way. This is also the first test model which describes O2·− production as the maximal rate of cytochrome (III) C reduction." @default.
- W1985728844 created "2016-06-24" @default.
- W1985728844 creator A5042111857 @default.
- W1985728844 creator A5065612999 @default.
- W1985728844 creator A5076320742 @default.
- W1985728844 date "1994-11-01" @default.
- W1985728844 modified "2023-10-16" @default.
- W1985728844 title "Measurement of superoxide anion production using maximal rate of cytochrome (III) C reduction in phorbol ester stimulated neutrophils, immobilised to microtiter plates" @default.
- W1985728844 cites W1606716377 @default.
- W1985728844 cites W1801129364 @default.
- W1985728844 cites W1966619356 @default.
- W1985728844 cites W1967039082 @default.
- W1985728844 cites W1968249471 @default.
- W1985728844 cites W1971648746 @default.
- W1985728844 cites W1973287769 @default.
- W1985728844 cites W1985042912 @default.
- W1985728844 cites W1985978000 @default.
- W1985728844 cites W1988181858 @default.
- W1985728844 cites W1999977358 @default.
- W1985728844 cites W2040217192 @default.
- W1985728844 cites W2078509464 @default.
- W1985728844 cites W2083322279 @default.
- W1985728844 cites W2105219152 @default.
- W1985728844 cites W2165214355 @default.
- W1985728844 cites W4214589643 @default.
- W1985728844 cites W4379136709 @default.
- W1985728844 doi "https://doi.org/10.1016/0006-2952(94)90595-9" @default.
- W1985728844 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7986208" @default.
- W1985728844 hasPublicationYear "1994" @default.
- W1985728844 type Work @default.
- W1985728844 sameAs 1985728844 @default.
- W1985728844 citedByCount "12" @default.
- W1985728844 countsByYear W19857288442012 @default.
- W1985728844 countsByYear W19857288442014 @default.
- W1985728844 countsByYear W19857288442016 @default.
- W1985728844 countsByYear W19857288442019 @default.
- W1985728844 crossrefType "journal-article" @default.
- W1985728844 hasAuthorship W1985728844A5042111857 @default.
- W1985728844 hasAuthorship W1985728844A5065612999 @default.
- W1985728844 hasAuthorship W1985728844A5076320742 @default.
- W1985728844 hasConcept C181199279 @default.
- W1985728844 hasConcept C185592680 @default.
- W1985728844 hasConcept C195794163 @default.
- W1985728844 hasConcept C2775838275 @default.
- W1985728844 hasConcept C2778004101 @default.
- W1985728844 hasConcept C2778810118 @default.
- W1985728844 hasConcept C2779084600 @default.
- W1985728844 hasConcept C2779661893 @default.
- W1985728844 hasConcept C2780043322 @default.
- W1985728844 hasConcept C2780795997 @default.
- W1985728844 hasConcept C28859421 @default.
- W1985728844 hasConcept C29311851 @default.
- W1985728844 hasConcept C43617362 @default.
- W1985728844 hasConcept C55493867 @default.
- W1985728844 hasConceptScore W1985728844C181199279 @default.
- W1985728844 hasConceptScore W1985728844C185592680 @default.
- W1985728844 hasConceptScore W1985728844C195794163 @default.
- W1985728844 hasConceptScore W1985728844C2775838275 @default.
- W1985728844 hasConceptScore W1985728844C2778004101 @default.
- W1985728844 hasConceptScore W1985728844C2778810118 @default.
- W1985728844 hasConceptScore W1985728844C2779084600 @default.
- W1985728844 hasConceptScore W1985728844C2779661893 @default.
- W1985728844 hasConceptScore W1985728844C2780043322 @default.
- W1985728844 hasConceptScore W1985728844C2780795997 @default.
- W1985728844 hasConceptScore W1985728844C28859421 @default.
- W1985728844 hasConceptScore W1985728844C29311851 @default.
- W1985728844 hasConceptScore W1985728844C43617362 @default.
- W1985728844 hasConceptScore W1985728844C55493867 @default.
- W1985728844 hasIssue "10" @default.
- W1985728844 hasLocation W19857288441 @default.
- W1985728844 hasLocation W19857288442 @default.
- W1985728844 hasOpenAccess W1985728844 @default.
- W1985728844 hasPrimaryLocation W19857288441 @default.
- W1985728844 hasRelatedWork W1907285155 @default.
- W1985728844 hasRelatedWork W1964508588 @default.
- W1985728844 hasRelatedWork W1998723340 @default.
- W1985728844 hasRelatedWork W2015873606 @default.
- W1985728844 hasRelatedWork W2036389950 @default.
- W1985728844 hasRelatedWork W2050734598 @default.
- W1985728844 hasRelatedWork W2060279471 @default.
- W1985728844 hasRelatedWork W2074678671 @default.
- W1985728844 hasRelatedWork W2171326444 @default.
- W1985728844 hasRelatedWork W2372270421 @default.
- W1985728844 hasVolume "48" @default.
- W1985728844 isParatext "false" @default.
- W1985728844 isRetracted "false" @default.
- W1985728844 magId "1985728844" @default.
- W1985728844 workType "article" @default.