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- W1985742009 abstract "We have previously shown an age-associated increase in unique CD4+CD8+ intestinal intraepithelial lymphocytes (i-IEL) in rats. To elucidate the potential causes of the increase in CD4+CD8+ i-IEL with age, we analyzed the specificity of the CD4+CD8+ i-IEL and influence of intestinal microflora on the increase in this subset in aged rats. The purified CD4+CD8+ i-IEL proliferated in response to DnaK [Escherichia colt (E. coli) HSP70] in the presence of mitomycin-c (MMC)-treated syngeneic, spleen cells. The proportion of CD4+CD8+ T cells in whole i-IEL were significantly increased in aged rats fed commercial (CL-2) diet but not in those fed home-made (purified) diet under conventional condition. No CD4+CD8+ i-IEL were detected in aged rats under germfree condition, irrespective of diet feeding. A larger number of Enterobacteriaceae, especially E. coli, were detected in the intestinal contents and feces from aged rats with CD4+CD8+ i-IEL compared with those from aged rats fed without CD4+CD8+ i-IEL. The unique CD4+CD8+ i-IEL population specific for E. coli HSP may be associated with long term exposure to intestinal E. coli in aged rats." @default.
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- W1985742009 date "1997-03-01" @default.
- W1985742009 modified "2023-09-26" @default.
- W1985742009 title "An unique CD4+CD8+ intestinal intraepithelial Lymphocyte Specific for DnaK (Escherichia coli HSP70) may be selected by intestinal microflora of rats" @default.
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- W1985742009 doi "https://doi.org/10.1016/s0171-2985(97)80071-x" @default.
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