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- W1985762275 abstract "An in-vitro system for infection of cat T lymphocytes with feline leukemia virus (FeLV) was developed. For establishment of this system, FeLV was inoculated in vitro into concanavalin-A stimulated primary lymphoid cells and long-term cultured T cells grown with interleukin-2 (IL-2) from various feline lympho-hematopoietic organs. During serial cultivation with IL-2, FeLV group-specific antigen (GSA) was detected 5-6 weeks after infection in the primary cultures, and 1 week after infection in the long-term cultures of T cells. The percentage of FeLV-GSA-positive cells gradually increased with passages and reached more than 80% in several cultures of lymphoid cells, that became persistently infected and produced FeLV. These FeLV-infected lymphoid cells expressed T-cell markers, but showed no obvious differences in growth or cytological characteristics from FeLV-free feline lymphoid cells during the observation period of 60 weeks." @default.
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- W1985762275 date "1987-01-01" @default.
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- W1985762275 title "Infection of feline lymphoid cells with feline leukemia virus in culture" @default.
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- W1985762275 doi "https://doi.org/10.1016/0145-2126(87)90091-9" @default.
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