FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985807785> ?p ?o ?g. }

• W1985807785 endingPage "303" @default.
• W1985807785 startingPage "296" @default.
• W1985807785 abstract "Coupling of carboxypeptidase with diazotized arsanilic acid specifically modifies a single tyrosyl residue. Yet, owing to the fact that the resultant azoTyr-248 can form an intramolecular chelate with zinc, two different circular dichroism probes result: azoTyr-248 itself and the azoTyr-248-Zn chelate. Both are environmentally sensitive and, characteristically, each can signal the same or different perturbations, as is apparent from circular dichroic spectra. This dual probe function greatly magnifies the scope of these chromophores in mapping the topography of the active center with respect to sites of interaction of inhibitors (or substrates). Titration of the azoenzyme with a series of synthetic, competitive inhibitors, e.g., L-benzylsuccinate, L-phenyllactate, and L-Phe, and with the pseudosubstrate, Gly-L-Tyr, in turn generates characteristic circular dichroic spectra. Their analysis yields a single binding constant for each of these agents, one molecule of each binding to the active center. Mixed inhibitions, as seen with beta-phenylpropionate and phenylacetate, resolved previously into competitive and noncompetitive components, are characterized by different spectral effects. Two molecules of these agents bind to the enzyme, consistent with both thermodynamic and enzymatic studies. The interactions leading to competitive and noncompetitive inhibition, respectively, can be recognized and assigned, based on the manner in which the extrema at 340 and 420 nm, reflecting azoTyr-248, and the negative 510-nm circular dichroism band, typical of its chelate with zinc, are affected and on the pH dependence of spectral and kinetic data. Certai4 noncompetitive inhibitors and modifiers induce yet other spectral features. Each probe is very sensitive to changes in its particular active center environment, though both can be relatively insensitive to inhibitors interacting at a distance from the active center." @default.
• W1985807785 created "2016-06-24" @default.
• W1985807785 creator A5015600123 @default.
• W1985807785 creator A5071054945 @default.
• W1985807785 creator A5073128489 @default.
• W1985807785 date "1976-01-01" @default.
• W1985807785 modified "2023-09-23" @default.
• W1985807785 title "Circular dichroism-inhibitor titrations of arsanilazotyrosine-248 carboxypeptidase A" @default.
• W1985807785 cites W117861301 @default.
• W1985807785 cites W1546504247 @default.
• W1985807785 cites W1968395904 @default.
• W1985807785 cites W1977956827 @default.
• W1985807785 cites W1991571763 @default.
• W1985807785 cites W1992985500 @default.
• W1985807785 cites W1994556899 @default.
• W1985807785 cites W2005252772 @default.
• W1985807785 cites W2005448691 @default.
• W1985807785 cites W2007571898 @default.
• W1985807785 cites W2009834336 @default.
• W1985807785 cites W2012602453 @default.
• W1985807785 cites W2013304453 @default.
• W1985807785 cites W2020087324 @default.
• W1985807785 cites W2021920356 @default.
• W1985807785 cites W2027492492 @default.
• W1985807785 cites W2040548886 @default.
• W1985807785 cites W2040825031 @default.
• W1985807785 cites W2045238017 @default.
• W1985807785 cites W2048193327 @default.
• W1985807785 cites W2051903006 @default.
• W1985807785 cites W2054933252 @default.
• W1985807785 cites W2058688637 @default.
• W1985807785 cites W2062932836 @default.
• W1985807785 cites W2070516655 @default.
• W1985807785 cites W2092449473 @default.
• W1985807785 cites W2100402242 @default.
• W1985807785 cites W2115926272 @default.
• W1985807785 cites W2129632725 @default.
• W1985807785 cites W2147468354 @default.
• W1985807785 cites W95764585 @default.
• W1985807785 doi "https://doi.org/10.1021/bi00647a009" @default.
• W1985807785 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1247518" @default.
• W1985807785 hasPublicationYear "1976" @default.
• W1985807785 type Work @default.
• W1985807785 sameAs 1985807785 @default.
• W1985807785 citedByCount "24" @default.
• W1985807785 crossrefType "journal-article" @default.
• W1985807785 hasAuthorship W1985807785A5015600123 @default.
• W1985807785 hasAuthorship W1985807785A5071054945 @default.
• W1985807785 hasAuthorship W1985807785A5073128489 @default.
• W1985807785 hasConcept C108477463 @default.
• W1985807785 hasConcept C133571119 @default.
• W1985807785 hasConcept C136398272 @default.
• W1985807785 hasConcept C142388413 @default.
• W1985807785 hasConcept C150757390 @default.
• W1985807785 hasConcept C178790620 @default.
• W1985807785 hasConcept C181199279 @default.
• W1985807785 hasConcept C184866935 @default.
• W1985807785 hasConcept C185592680 @default.
• W1985807785 hasConcept C192468462 @default.
• W1985807785 hasConcept C197404232 @default.
• W1985807785 hasConcept C2779811187 @default.
• W1985807785 hasConcept C32909587 @default.
• W1985807785 hasConcept C535196362 @default.
• W1985807785 hasConcept C71240020 @default.
• W1985807785 hasConcept C75473681 @default.
• W1985807785 hasConcept C8010536 @default.
• W1985807785 hasConceptScore W1985807785C108477463 @default.
• W1985807785 hasConceptScore W1985807785C133571119 @default.
• W1985807785 hasConceptScore W1985807785C136398272 @default.
• W1985807785 hasConceptScore W1985807785C142388413 @default.
• W1985807785 hasConceptScore W1985807785C150757390 @default.
• W1985807785 hasConceptScore W1985807785C178790620 @default.
• W1985807785 hasConceptScore W1985807785C181199279 @default.
• W1985807785 hasConceptScore W1985807785C184866935 @default.
• W1985807785 hasConceptScore W1985807785C185592680 @default.
• W1985807785 hasConceptScore W1985807785C192468462 @default.
• W1985807785 hasConceptScore W1985807785C197404232 @default.
• W1985807785 hasConceptScore W1985807785C2779811187 @default.
• W1985807785 hasConceptScore W1985807785C32909587 @default.
• W1985807785 hasConceptScore W1985807785C535196362 @default.
• W1985807785 hasConceptScore W1985807785C71240020 @default.
• W1985807785 hasConceptScore W1985807785C75473681 @default.
• W1985807785 hasConceptScore W1985807785C8010536 @default.
• W1985807785 hasIssue "2" @default.
• W1985807785 hasLocation W19858077851 @default.
• W1985807785 hasLocation W19858077852 @default.
• W1985807785 hasOpenAccess W1985807785 @default.
• W1985807785 hasPrimaryLocation W19858077851 @default.
• W1985807785 hasRelatedWork W176342255 @default.
• W1985807785 hasRelatedWork W1982364997 @default.
• W1985807785 hasRelatedWork W1997284210 @default.
• W1985807785 hasRelatedWork W2023079816 @default.
• W1985807785 hasRelatedWork W2028036603 @default.
• W1985807785 hasRelatedWork W2041521114 @default.
• W1985807785 hasRelatedWork W2048490182 @default.
• W1985807785 hasRelatedWork W2104071083 @default.
• W1985807785 hasRelatedWork W2393994965 @default.
• W1985807785 hasRelatedWork W2409634677 @default.

• next