FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985809249> ?p ?o ?g. }

• W1985809249 abstract "Abstract Background Infection with human immunodeficiency virus type-1 (HIV)-1 leads to some form of HIV-1-associated neurocognitive disorders (HAND) in approximately half of the cases. The mechanisms by which astrocytes contribute to HIV-1-associated dementia (HAD), the most severe form of HAND, still remain unresolved. HIV-1-encephalitis (HIVE), a pathological correlate of HAD, affects an estimated 9-11% of the HIV-1-infected population. Our laboratory has previously demonstrated that HIVE brain tissues show significant upregulation of CD38, an enzyme involved in calcium signaling, in astrocytes. We also reported an increase in CD38 expression in interleukin (IL)-1β-activated astrocytes. In the present investigation, we studied regulatory mechanisms of CD38 gene expression in astrocytes activated with HIV-1-relevant stimuli. We also investigated the role of mitogen-activated protein kinases (MAPKs) and nuclear factor (NF)-κB in astrocyte CD38 regulation. Methods Cultured human astrocytes were transfected with HIV-1 YU-2 proviral clone and levels of CD38 mRNA and protein were measured by real-time PCR gene expression assay, western blot analysis and immunostaining. Astrocyte activation by viral transfection was determined by analyzing proinflammatory chemokine levels using ELISA. To evaluate the roles of MAPKs and NF-κB in CD38 regulation, astrocytes were treated with MAPK inhibitors (SB203580, SP600125, U0126), NF-κB interfering peptide (SN50) or transfected with dominant negative IκBα mutant (IκBαM) prior to IL-1β activation. CD38 gene expression and CD38 ADP-ribosyl cyclase activity assays were performed to analyze alterations in CD38 levels and function, respectively. Results HIV-1 YU-2 -transfection significantly increased CD38 mRNA and protein expression in astrocytes (p < 0.01) in a dose-dependent manner and induced astrocyte activation. IL-β-activation of HIV-1 YU-2 -transfected astrocytes significantly increased HIV-1 gene expression (p < 0.001). Treatment with MAPK inhibitors or NF-κB inhibitor SN50 abrogated IL-1β-induced CD38 expression and activity in astrocytes without altering basal CD38 levels (p < 0.001). IκBαM transfection also significantly inhibited IL-1β-mediated increases in CD38 expression and activity in astrocytes (p < 0.001). Conclusion The present findings demonstrate a direct involvement of HIV-1 and virus-induced proinflammatory stimuli in regulating astrocyte-CD38 levels. HIV-1 YU-2 -transfection effectively induced HIV-1 p 24 protein expression and activated astrocytes to upregulate CCL2, CXCL8 and CD38. In astrocytes, IL-1β-induced increases in CD38 levels were regulated through the MAPK signaling pathway and by the transcription factor NF-κB. Future studies may be directed towards understanding the role of CD38 in response to infection and thus its role in HAND." @default.
• W1985809249 created "2016-06-24" @default.
• W1985809249 creator A5016364609 @default.
• W1985809249 creator A5017897338 @default.
• W1985809249 creator A5031598979 @default.
• W1985809249 creator A5064953433 @default.
• W1985809249 creator A5070922052 @default.
• W1985809249 creator A5073472235 @default.
• W1985809249 creator A5073817913 @default.
• W1985809249 date "2011-10-25" @default.
• W1985809249 modified "2023-09-27" @default.
• W1985809249 title "HIV-1 and IL-1β regulate astrocytic CD38 through mitogen-activated protein kinases and nuclear factor-κB signaling mechanisms" @default.
• W1985809249 cites W103311651 @default.
• W1985809249 cites W1488696767 @default.
• W1985809249 cites W1492606093 @default.
• W1985809249 cites W1555919459 @default.
• W1985809249 cites W1580586916 @default.
• W1985809249 cites W1700464050 @default.
• W1985809249 cites W1870780415 @default.
• W1985809249 cites W1925039798 @default.
• W1985809249 cites W1965911531 @default.
• W1985809249 cites W1967436988 @default.
• W1985809249 cites W1971091859 @default.
• W1985809249 cites W1971213778 @default.
• W1985809249 cites W1971704375 @default.
• W1985809249 cites W1974305753 @default.
• W1985809249 cites W1977231617 @default.
• W1985809249 cites W1980969896 @default.
• W1985809249 cites W1981376840 @default.
• W1985809249 cites W1983661921 @default.
• W1985809249 cites W1987171087 @default.
• W1985809249 cites W1989463753 @default.
• W1985809249 cites W1992584725 @default.
• W1985809249 cites W1993327334 @default.
• W1985809249 cites W1996405087 @default.
• W1985809249 cites W1997412988 @default.
• W1985809249 cites W2000250827 @default.
• W1985809249 cites W2004997979 @default.
• W1985809249 cites W2006782547 @default.
• W1985809249 cites W2009969882 @default.
• W1985809249 cites W2010358244 @default.
• W1985809249 cites W2016983155 @default.
• W1985809249 cites W2018783500 @default.
• W1985809249 cites W2029894447 @default.
• W1985809249 cites W2036542628 @default.
• W1985809249 cites W2040554400 @default.
• W1985809249 cites W2041476640 @default.
• W1985809249 cites W2043001990 @default.
• W1985809249 cites W2046088592 @default.
• W1985809249 cites W2046371940 @default.
• W1985809249 cites W2048130546 @default.
• W1985809249 cites W2054209007 @default.
• W1985809249 cites W2055270900 @default.
• W1985809249 cites W2059830137 @default.
• W1985809249 cites W2059893490 @default.
• W1985809249 cites W2067150920 @default.
• W1985809249 cites W2069499548 @default.
• W1985809249 cites W2070169817 @default.
• W1985809249 cites W2072126783 @default.
• W1985809249 cites W2074661552 @default.
• W1985809249 cites W2074701257 @default.
• W1985809249 cites W2076520858 @default.
• W1985809249 cites W2079320514 @default.
• W1985809249 cites W2087416436 @default.
• W1985809249 cites W2090458882 @default.
• W1985809249 cites W2093234204 @default.
• W1985809249 cites W2109107050 @default.
• W1985809249 cites W2110683265 @default.
• W1985809249 cites W2112945948 @default.
• W1985809249 cites W2115168263 @default.
• W1985809249 cites W2137012634 @default.
• W1985809249 cites W2137319294 @default.
• W1985809249 cites W2138902434 @default.
• W1985809249 cites W2144335184 @default.
• W1985809249 cites W2145362945 @default.
• W1985809249 cites W2147638621 @default.
• W1985809249 cites W2148682568 @default.
• W1985809249 cites W2153549293 @default.
• W1985809249 cites W2165311084 @default.
• W1985809249 cites W2168626405 @default.
• W1985809249 cites W4249508584 @default.
• W1985809249 cites W4313325039 @default.
• W1985809249 doi "https://doi.org/10.1186/1742-2094-8-145" @default.
• W1985809249 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3247131" @default.
• W1985809249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22027397" @default.
• W1985809249 hasPublicationYear "2011" @default.
• W1985809249 type Work @default.
• W1985809249 sameAs 1985809249 @default.
• W1985809249 citedByCount "33" @default.
• W1985809249 countsByYear W19858092492012 @default.
• W1985809249 countsByYear W19858092492013 @default.
• W1985809249 countsByYear W19858092492014 @default.
• W1985809249 countsByYear W19858092492015 @default.
• W1985809249 countsByYear W19858092492016 @default.
• W1985809249 countsByYear W19858092492017 @default.
• W1985809249 countsByYear W19858092492018 @default.
• W1985809249 countsByYear W19858092492019 @default.
• W1985809249 countsByYear W19858092492020 @default.
• W1985809249 countsByYear W19858092492021 @default.
• W1985809249 countsByYear W19858092492022 @default.

• next