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• W1985821073 abstract "Pregnenolone-binding activity isolated from the cytosol of the guinea pig adrenal cortex appears to correspond to a M r 34,000 protein when examined by SDS-polyacrylamide gel electrophoresis during different stages of purification. To verify this finding the M r 34,000 protein band was eluted from the SDS gel and used to generate a polyclonal antibody. Immobilized anti 34,000 IgG on protein A-Sepharose was found to extract pregnenolone-binding activity from solution in contrast to pre-immune IgG and an antibody raised against a M r 30,000 protein isolated simultaneously. In addition, protein eluted from the protein A-anti 34,000 IgG complex exhibited the expected molecular weight of 34,000 when examined on an SDS gel. These results, thus, confirm that the pregnenolone-binding protein is indeed a protein of M r 34,000." @default.
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• W1985821073 date "1988-01-01" @default.
• W1985821073 modified "2023-09-25" @default.
• W1985821073 title "Adrenocortical pregnenolone-binding protein: Identification and antibody development" @default.
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• W1985821073 doi "https://doi.org/10.1016/0006-291x(88)90542-6" @default.
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