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• W1985867932 abstract "An in vitro model was developed to assay the effects of static stretching on soluble elastin (tropoelastin, TE) synthesis by porcine aorta smooth muscle cells (SMC).Culture dishes containing SMC adherent to the deformable bottoms were placed over hard convex templates.Stress was applied by placing a weight on the dish covers.Measurement of TE was accomplished by a specific ELISA assay.With this model we demonstrated reproducible stimulation of TE synthesis by stretched SMC.Significant results (161.4% of control; p = 0.003 by Student's t-test) were obtained by stretching passage 2 SMC for 3 days with a medium change after the first 18-24hours, use of 5% newborn calf serum in cultures during stretching, and 50-g weight.DNA content in stretched cultures did not increase over control values.Thus, stretching alone did not cause hyperplasia or hyperploidy in these SMC and, in the absence of other vascular cell elements, was sufficient to increase production of this extracellular matrix protein.Transduction of mechanical force into elastin gene expression by SMC may contribute to the development of thickened arterial tunica media characteristic of hypertensive vessels." @default.
• W1985867932 created "2016-06-24" @default.
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• W1985867932 date "1990-07-01" @default.
• W1985867932 modified "2023-10-18" @default.
• W1985867932 title "Effect of Static Stretching on Elastin Production by Porcine Aortic Smooth Muscle Cells" @default.
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• W1985867932 doi "https://doi.org/10.1016/s0934-8832(11)80163-0" @default.
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