Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985900979> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1985900979 abstract "Phosphofructokinase-1 from Saccharomyces cerevisiae is composed of two types of subunits, alpha and beta. Subunit-specific monoclonal antibodies were raised to elucidate structural and functional properties of both subunits. One monoclonal antibody, alpha-F3, binds to an epitope either at the C-terminal or at the N-terminal part of the alpha-polypeptide chain. By screening a heptapeptide library with this monoclonal antibody, a set of heptapeptides was selected, which contained the consensus sequences D-A-F and D-S-F. Two heptapeptides with these motifs were synthesized in order assess their capacity to inhibit the binding of antibody alpha-F3 to native phosphofructokinase-1. The peptide G-I-K-D-A-F-L inhibited the binding more strongly (IC50 = 1.5 microM) than the peptide A-P-W-H-D-S-F (IC50 = 33.3 microM). Sequence matching revealed the presence of the D-A-F motif in the polypeptide chain of phosphofructokinase-1 at amino acid position 172-174. As a control, the nonapeptide A-P-T-S-K-D-A-F-L which corresponds to the sequence of the putative epitope was tested in the inhibition assay. In view of the high inhibitory capacity (IC50 = 0.3 microM) it was concluded that this nonapeptide represents the continuous epitope of phosphofructokinase-1 that is recognized by antibody alpha-F3." @default.
- W1985900979 created "2016-06-24" @default.
- W1985900979 creator A5027247545 @default.
- W1985900979 creator A5036339128 @default.
- W1985900979 creator A5069577467 @default.
- W1985900979 creator A5080867341 @default.
- W1985900979 date "1999-01-01" @default.
- W1985900979 modified "2023-09-25" @default.
- W1985900979 title "Epitope mapping of a monoclonal antibody directed against the α-subunit of phosphofructokinase-1 fromSaccharomyces cerevisiae by screening phage display libraries" @default.
- W1985900979 cites W1255694588 @default.
- W1985900979 cites W1522829305 @default.
- W1985900979 cites W1591942886 @default.
- W1985900979 cites W1638961546 @default.
- W1985900979 cites W1846628205 @default.
- W1985900979 cites W1996427305 @default.
- W1985900979 cites W1998522293 @default.
- W1985900979 cites W2021144157 @default.
- W1985900979 cites W2076415952 @default.
- W1985900979 cites W2080657914 @default.
- W1985900979 cites W2084429764 @default.
- W1985900979 cites W2100837269 @default.
- W1985900979 cites W4248456746 @default.
- W1985900979 doi "https://doi.org/10.1002/(sici)1099-1352(199901/02)12:1<33::aid-jmr442>3.0.co;2-m" @default.
- W1985900979 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10398394" @default.
- W1985900979 hasPublicationYear "1999" @default.
- W1985900979 type Work @default.
- W1985900979 sameAs 1985900979 @default.
- W1985900979 citedByCount "1" @default.
- W1985900979 crossrefType "journal-article" @default.
- W1985900979 hasAuthorship W1985900979A5027247545 @default.
- W1985900979 hasAuthorship W1985900979A5036339128 @default.
- W1985900979 hasAuthorship W1985900979A5069577467 @default.
- W1985900979 hasAuthorship W1985900979A5080867341 @default.
- W1985900979 hasConcept C104292427 @default.
- W1985900979 hasConcept C104317684 @default.
- W1985900979 hasConcept C122245378 @default.
- W1985900979 hasConcept C153911025 @default.
- W1985900979 hasConcept C159654299 @default.
- W1985900979 hasConcept C167625842 @default.
- W1985900979 hasConcept C181199279 @default.
- W1985900979 hasConcept C185592680 @default.
- W1985900979 hasConcept C186268636 @default.
- W1985900979 hasConcept C195616568 @default.
- W1985900979 hasConcept C20251656 @default.
- W1985900979 hasConcept C2779281246 @default.
- W1985900979 hasConcept C2781241564 @default.
- W1985900979 hasConcept C542903549 @default.
- W1985900979 hasConcept C54355233 @default.
- W1985900979 hasConcept C55493867 @default.
- W1985900979 hasConcept C78598654 @default.
- W1985900979 hasConcept C86803240 @default.
- W1985900979 hasConceptScore W1985900979C104292427 @default.
- W1985900979 hasConceptScore W1985900979C104317684 @default.
- W1985900979 hasConceptScore W1985900979C122245378 @default.
- W1985900979 hasConceptScore W1985900979C153911025 @default.
- W1985900979 hasConceptScore W1985900979C159654299 @default.
- W1985900979 hasConceptScore W1985900979C167625842 @default.
- W1985900979 hasConceptScore W1985900979C181199279 @default.
- W1985900979 hasConceptScore W1985900979C185592680 @default.
- W1985900979 hasConceptScore W1985900979C186268636 @default.
- W1985900979 hasConceptScore W1985900979C195616568 @default.
- W1985900979 hasConceptScore W1985900979C20251656 @default.
- W1985900979 hasConceptScore W1985900979C2779281246 @default.
- W1985900979 hasConceptScore W1985900979C2781241564 @default.
- W1985900979 hasConceptScore W1985900979C542903549 @default.
- W1985900979 hasConceptScore W1985900979C54355233 @default.
- W1985900979 hasConceptScore W1985900979C55493867 @default.
- W1985900979 hasConceptScore W1985900979C78598654 @default.
- W1985900979 hasConceptScore W1985900979C86803240 @default.
- W1985900979 hasLocation W19859009791 @default.
- W1985900979 hasLocation W19859009792 @default.
- W1985900979 hasOpenAccess W1985900979 @default.
- W1985900979 hasPrimaryLocation W19859009791 @default.
- W1985900979 hasRelatedWork W2001216269 @default.
- W1985900979 hasRelatedWork W2012958292 @default.
- W1985900979 hasRelatedWork W2027698777 @default.
- W1985900979 hasRelatedWork W2061470026 @default.
- W1985900979 hasRelatedWork W2139816899 @default.
- W1985900979 hasRelatedWork W2164532875 @default.
- W1985900979 hasRelatedWork W2171458163 @default.
- W1985900979 hasRelatedWork W2353451622 @default.
- W1985900979 hasRelatedWork W2374075475 @default.
- W1985900979 hasRelatedWork W3047621897 @default.
- W1985900979 isParatext "false" @default.
- W1985900979 isRetracted "false" @default.
- W1985900979 magId "1985900979" @default.
- W1985900979 workType "article" @default.