FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1985909114> ?p ?o ?g. }

• W1985909114 endingPage "144" @default.
• W1985909114 startingPage "133" @default.
• W1985909114 abstract "Male germline stem cells (mGSCs) are stem cells present in male testis responsible for spermatogenesis during their whole life. Studies have shown that mGSCs can be derived in vitro and resemble embryonic stem cells (ESCs) properties both in the mouse and humans. However, little is know about these cells in domestic animals. Here we report the first successful establishment of goat GSCs derived from 2–5-month fetal testis, and developmental potential assay of these cells both in vitro and in vivo. These cells express pluripotent markers such as Oct4, Sox2, C-myc, and Tert when cultured as human ESCs conditions. Embryoid bodies (EBs) formed by goat mGSCs were induced with 2 × 10−6 M retinoic acid (RA). Immunofluorescence analysis showed that some cells inside of the EBs were positive for meiosis marker—SCP3, STRA8, and germ cell marker—VASA, and haploid marker-FE-J1, PRM1, indicating their germ cell lineage differentiation. Some cells become elongated sperm-like cells after induction. Approximately 34.88% (30/86) embryos showed cleavage and four embryos were cultured on murine fibroblast feeder and formed small embryonic stem like colonies. However, most stalled at four-cell stage after intracytoplasmic sperm injection (ICSI) of these cells. Transplantation of DAPI labeled mGSCs into the seminiferous tubules of busulfan-treated mice, and showed that mGSCs can colonize, self-renew, and differentiate into germ cells. Thus, we have established a goat GSC cell line and these cells could be differentiated into sperm-like cells in vivo and sperms in vitro, providing a promising platform for generation of transgenic goat for production of specific humanized proteins." @default.
• W1985909114 created "2016-06-24" @default.
• W1985909114 creator A5000432967 @default.
• W1985909114 creator A5001954462 @default.
• W1985909114 creator A5004032681 @default.
• W1985909114 creator A5028296024 @default.
• W1985909114 creator A5034247665 @default.
• W1985909114 creator A5045856174 @default.
• W1985909114 creator A5046823511 @default.
• W1985909114 creator A5058450296 @default.
• W1985909114 creator A5058764792 @default.
• W1985909114 date "2011-04-01" @default.
• W1985909114 modified "2023-09-26" @default.
• W1985909114 title "Pluripotent Male Germline Stem Cells from Goat Fetal Testis and Their Survival in Mouse Testis" @default.
• W1985909114 cites W1584801101 @default.
• W1985909114 cites W1974652594 @default.
• W1985909114 cites W1977135603 @default.
• W1985909114 cites W1979700611 @default.
• W1985909114 cites W1983285107 @default.
• W1985909114 cites W1992242742 @default.
• W1985909114 cites W1993491850 @default.
• W1985909114 cites W1995140011 @default.
• W1985909114 cites W1999533529 @default.
• W1985909114 cites W2013518855 @default.
• W1985909114 cites W2016789158 @default.
• W1985909114 cites W2027011084 @default.
• W1985909114 cites W2030018241 @default.
• W1985909114 cites W2032480797 @default.
• W1985909114 cites W2033042516 @default.
• W1985909114 cites W2038752568 @default.
• W1985909114 cites W2039191618 @default.
• W1985909114 cites W2045899482 @default.
• W1985909114 cites W2054939637 @default.
• W1985909114 cites W2064579441 @default.
• W1985909114 cites W2067924039 @default.
• W1985909114 cites W2078337444 @default.
• W1985909114 cites W2081023675 @default.
• W1985909114 cites W2085171607 @default.
• W1985909114 cites W2086470708 @default.
• W1985909114 cites W2092086404 @default.
• W1985909114 cites W2095834286 @default.
• W1985909114 cites W2097370341 @default.
• W1985909114 cites W2113933705 @default.
• W1985909114 cites W2120557292 @default.
• W1985909114 cites W2128352374 @default.
• W1985909114 cites W2128433459 @default.
• W1985909114 cites W2129682283 @default.
• W1985909114 cites W2133660368 @default.
• W1985909114 cites W2135373970 @default.
• W1985909114 cites W2139789628 @default.
• W1985909114 cites W2143023822 @default.
• W1985909114 cites W2143526409 @default.
• W1985909114 cites W2144874927 @default.
• W1985909114 cites W2147898515 @default.
• W1985909114 cites W2148661975 @default.
• W1985909114 cites W2154269795 @default.
• W1985909114 cites W2156321367 @default.
• W1985909114 cites W2156537168 @default.
• W1985909114 cites W2164793314 @default.
• W1985909114 cites W2165085446 @default.
• W1985909114 cites W2165209578 @default.
• W1985909114 cites W2165892496 @default.
• W1985909114 doi "https://doi.org/10.1089/cell.2010.0047" @default.
• W1985909114 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21473690" @default.
• W1985909114 hasPublicationYear "2011" @default.
• W1985909114 type Work @default.
• W1985909114 sameAs 1985909114 @default.
• W1985909114 citedByCount "36" @default.
• W1985909114 countsByYear W19859091142012 @default.
• W1985909114 countsByYear W19859091142013 @default.
• W1985909114 countsByYear W19859091142014 @default.
• W1985909114 countsByYear W19859091142015 @default.
• W1985909114 countsByYear W19859091142016 @default.
• W1985909114 countsByYear W19859091142017 @default.
• W1985909114 countsByYear W19859091142018 @default.
• W1985909114 countsByYear W19859091142020 @default.
• W1985909114 crossrefType "journal-article" @default.
• W1985909114 hasAuthorship W1985909114A5000432967 @default.
• W1985909114 hasAuthorship W1985909114A5001954462 @default.
• W1985909114 hasAuthorship W1985909114A5004032681 @default.
• W1985909114 hasAuthorship W1985909114A5028296024 @default.
• W1985909114 hasAuthorship W1985909114A5034247665 @default.
• W1985909114 hasAuthorship W1985909114A5045856174 @default.
• W1985909114 hasAuthorship W1985909114A5046823511 @default.
• W1985909114 hasAuthorship W1985909114A5058450296 @default.
• W1985909114 hasAuthorship W1985909114A5058764792 @default.
• W1985909114 hasConcept C104317684 @default.
• W1985909114 hasConcept C107459253 @default.
• W1985909114 hasConcept C136302470 @default.
• W1985909114 hasConcept C138851942 @default.
• W1985909114 hasConcept C143948831 @default.
• W1985909114 hasConcept C145103041 @default.
• W1985909114 hasConcept C153911025 @default.
• W1985909114 hasConcept C16685009 @default.
• W1985909114 hasConcept C20875687 @default.
• W1985909114 hasConcept C26805974 @default.
• W1985909114 hasConcept C2777933648 @default.
• W1985909114 hasConcept C2779122487 @default.

• next