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• W1985986400 endingPage "2360" @default.
• W1985986400 startingPage "2351" @default.
• W1985986400 abstract "Sequence analysis of 236 promoters recognized by the Bacillus subtilis sigma A-RNA polymerase reveals an extended promoter structure. The most highly conserved bases include the -35 and -10 hexanucleotide core elements and a TG dinucleotide at position -15, -14. In addition, several weakly conserved A and T residues are present upstream of the -35 region. Analysis of dinucleotide composition reveals A2- and T2-rich sequences in the upstream promoter region (-36 to -70) which are phased with the DNA helix: An tracts are common near -43, -54 and -65; Tn tracts predominate at the intervening positions. When compared with larger regions of the genome, upstream promoter regions have an excess of An and Tn sequences for n > 4. These data indicate that an RNA polymerase binding site affects DNA sequence as far upstream as -70. This sequence conservation is discussed in light of recent evidence that the alpha subunits of the polymerase core bind DNA and that the promoter may wrap around RNA polymerase." @default.
• W1985986400 created "2016-06-24" @default.
• W1985986400 creator A5032712331 @default.
• W1985986400 date "1995-01-01" @default.
• W1985986400 modified "2023-10-11" @default.
• W1985986400 title "Compilation and analysus of<i>Bacillus Subtilis</i>σ^A-dependent promoter sequences: evidence for extended contact between RNA polymerse and upstream promoter DNA" @default.
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