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- W1985994712 abstract "There have been numerous proposals suggesting that whole-culture methods – in which all cells in a growing culture are treated identically – can synchronize cells. An explicit defense of these methods has been presented (Spellman and Sherlock, this issue, pp. 270–273, DOI:10.1016/j.tibtech.2004.04.010). Here, this defense of whole-culture ‘synchronization’ is subjected to a critical evaluation leading to the conclusion that whole-culture synchronization cannot synchronize cells – at all. Whole-culture methods cannot produce a set of cells that reflects the size and genome composition of cells of any particular cell-cycle age during the normal cell cycle. Thus, in addition to the well-recognized problem of artifacts, it is proposed that experiments using whole-culture treatments (usually starvation or inhibition methods) are not suitable for cell-cycle analysis because these methods do not produce a synchronized culture." @default.
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- W1985994712 date "2004-06-01" @default.
- W1985994712 modified "2023-09-28" @default.
- W1985994712 title "Rejoinder: whole-culture synchronization cannot, and does not, synchronize cells" @default.
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- W1985994712 doi "https://doi.org/10.1016/j.tibtech.2004.04.011" @default.
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